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Published on: October 14, 2011
Genome organization of membrane-containing bacteriophage PRD1
J K Bamford1, A L Hänninen, T M Pakula
1Department of Genetics, University of Helsinki, Finland.
Abstract:
We have determined the nucleotide sequence of the late region (11 kbp) of the lipid-containing bacteriophage PRD1. Gene localization was carried out by complementing nonsense phage mutants with genomic clones containing specific reading frames. The localization was confirmed by sequencing the N-termini of isolated gene products as well as sequencing the N-termini of tryptic fragments of the phage membrane-associated proteins. This, with the previously obtained sequence of the early regions, allowed us to organize most of the phage genes in the phage genome.
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