Related Experiment Video
Updated: Jul 4, 2026
Chick ex ovo Culture and ex ovo CAM Assay: How it Really Works
Published on: November 30, 2009
[Experimental study of Solanum nigrum on inhibiting angiogenesis in chick chorioallantoic membrane]
Yang Xu1, Rui-Le Pan, Qi Chang
1Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100094, China. xuyang65@hotmail.com
Objective:
To study the inhibitory effect of Solanum nigrum on angiogenesis.
Method:
We examined the effects of S. nigrum on angiogenesis in the chick chorioallantoic membrane (CAM) model. On day 7 of chick embryo incubation, three concentrations of S. nigrum aqueous extracts were applied to CAMs, and their effects were evaluated on day 9.
Result:
The angiogenesis area was significantly smaller in the CAM treated with S. nigrum than that in the control group (P < 0.001). Pathology analysis indicated that less angiogenesis occurred in the tissue of CAM under the filter paper treated with S. nigrum and the structure of large arteries was destroyed. The surrounding CAM showed a few angiogenesis formation. However, in the control group, a number of angiogenesis were observed.
Conclusion:
S. nigrum could inhibit the angiogenesis on CAM.
