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[Determination of content of saikoside in Bupleurum Chinense from different sources]
Yuan-Yuan Li1, Xue-Mei Qin, Yu-Qing Wang
1School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China.
Objective:
To determine the contents of saikosaponin a, d and saikosaponins in Radix Bupleuri from different and assess their quality.
Method:
Saikosaponin a and d were used as the chemical reference substance to establish RP-HPLC and visible-spectrophotometry methods for determination of contents of saikosaponin a, d and saikosaponins.
Result:
The quality of B. chinense was better than that of B. scozonerifolium and B smishii; The contents of a, saikosaponin d and total saikosaponin in B. chinense were 0.100%-0.330%, 0.200%-0.400% and 1.00%-2.00% , respectively.
Conclusion:
It is to suggest that the minimal contents requirements of saikosaponin a, d and saikosaponins in B. chinense should be set at 0. 100% , 0.200% and 1.00% , respectively; the best suitable planting areas are Taigu in the middle of Shanxi province, Ruicheng, Pinglu and Wanrong in the south of Shanxi province, and Longxi, Lixian in Gansu province.