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[Enriching of total flavonoids from Herba Leonuri with polyamide and macroporous resin]
Dan-Ni Yu1, De-Sheng Xu, Yi Feng
1China Pharmaceutical University, Nanjing 210009, China.
Objective:
To study the enriching method of total flavonoid from Herba Leonuri with polyamide and macroporous resin.
Method:
Seven enriching and purifying methods were compared with the yield and purity as indexes. The method of enriching with polyamide and macroporous resin was confirmed and the process of purifying was determined by orthogonal design.
Result:
D101 resin is packed by wet method, the ratio of diameter to height is 1:7. After mixed with the extract liquids, the weight of wet resin increased to 3 times of the dry resin. Evaporated the wet resin to dryness, mixed well with a little of 95% ethanol and dry polyamide powder, evaporated them to dryness again. Elute with deionized water until the effluent being colourless, then loaded it on the macroporous adsorptive resin, elute with 50% ethanol, the volume of effluents was collected to 7 times of the column volume. The purity of total flavonoids reached to 23%, while the diversion rate from raw Herba Leonuri was to 69%.
Conclusion:
The process is simple and convenient, and the regeneration of resin is easy, which has a good application foreground.
