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Enzymatic Synthesis of Epoxidized Metabolites of Docosahexaenoic, Eicosapentaenoic, and Arachidonic Acids
Published on: June 28, 2019
Enzymatic synthesis of astaxanthin n-octanoic acid esters
Masahiro Nakao1, Motoo Sumida, Kenji Katano
1Institute for Advanced Technology, Technological Development Center, Suntory Ltd., Yamazaki, Mishima-gun, Osaka, Japan.
Abstract:
We examined the enzymatic synthesis of astaxanthin n-octanoic acid esters. Carriers for the immobilized enzyme and reaction conditions such as water content, reaction temperature, and time were examined using Candida cylindracea lipase (Lipase OF). Lipase OF) immobilized by a hydrophobic anion exchange resin (10% w/w content of lipase) gave the best yield in the esterification reaction of astaxanthin. Two milligrams of astaxanthin per 750 microL tri-n-octanoin (ca. 0.3%) was optimum because of the low solubility of tri-n-octanoin. The esters were obtained in a yield of 36.4% under the optimal reaction conditions.
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