Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
iChip01:24

iChip

The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Long COVID symptoms before and 3-35 months after SARS-CoV-2 infection in a Norwegian prospective cohort study, symptoms over the pandemic, and different virus variants.

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases·2026
Same author

Acute COVID-19 severity and impaired cognitive function up to 32 months after diagnosis: an observational study.

BMC medicine·2026
Same author

Multimodal epigenetic and enhancer network remodeling shape the transcriptional landscape of human beige adipocytes.

Communications biology·2026
Same author

Major waves of H2A.Z incorporation during mouse oogenesis and preimplantation embryo development.

Nature communications·2025
Same author

Broad H3K4me3 domains support oocyte genome silencing and maturation but are dispensable for repression in early embryos.

Development (Cambridge, England)·2025
Same author

Loss of multilevel 3D genome organization during breast cancer progression.

Genome research·2025

Related Experiment Video

Updated: Jul 4, 2026

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos
11:02

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos

Published on: April 29, 2011

A rapid micro chromatin immunoprecipitation assay (microChIP).

John Arne Dahl1, Philippe Collas

  • 1Department of Biochemistry, Faculty of Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo 0317, Norway.

Nature Protocols
|June 10, 2008
PubMed
Summary

A new micro chromatin immunoprecipitation (microChIP) protocol enables protein-DNA interaction studies using rare cells or small biopsies. This 1-day method streamlines chromatin immunoprecipitation assays for faster, more accessible research.

More Related Videos

Chromatin Immunoprecipitation Assay Using Micrococcal Nucleases in Mammalian Cells
11:42

Chromatin Immunoprecipitation Assay Using Micrococcal Nucleases in Mammalian Cells

Published on: May 10, 2019

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing (ChIP-seq)
09:52

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing (ChIP-seq)

Published on: April 19, 2013

Related Experiment Videos

Last Updated: Jul 4, 2026

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos
11:02

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos

Published on: April 29, 2011

Chromatin Immunoprecipitation Assay Using Micrococcal Nucleases in Mammalian Cells
11:42

Chromatin Immunoprecipitation Assay Using Micrococcal Nucleases in Mammalian Cells

Published on: May 10, 2019

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing (ChIP-seq)
09:52

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing (ChIP-seq)

Published on: April 19, 2013

Area of Science:

  • Molecular Biology
  • Genomics
  • Epigenetics

Background:

  • Protein-DNA interactions are crucial for nuclear functions.
  • Chromatin immunoprecipitation (ChIP) is a key method for studying these interactions.
  • Existing ChIP methods often require large cell numbers or extensive time, limiting their use.

Purpose of the Study:

  • To develop a streamlined, rapid chromatin immunoprecipitation protocol.
  • To enable ChIP analysis from limited cell populations and small tissue samples.
  • To optimize ChIP for high-throughput analysis of protein-DNA interactions.

Main Methods:

  • A novel 1-day micro chromatin immunoprecipitation (microChIP) protocol was established.
  • The protocol was optimized for small cell numbers (1,000 cells) and small tissue volumes (1 mm³).
  • Modifications included adjustments to cross-linking and chromatin preparation steps.

Main Results:

  • The microChIP protocol allows up to 16 parallel immunoprecipitations in approximately 8 hours.
  • It is suitable for analyzing histone and/or transcription factor binding.
  • The method effectively monitors protein association with multiple genomic sites using as few as 100 cells.

Conclusions:

  • MicroChIP significantly reduces the cell input and time required for ChIP assays.
  • This technique expands the applicability of ChIP to rare cell samples and small biopsies.
  • The optimized protocol facilitates efficient and accessible study of protein-DNA interactions.