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Related Experiment Video

Updated: Jul 4, 2026

Stepwise Dosing Protocol for Increased Throughput in Label-Free Impedance-Based GPCR Assays
06:13

Stepwise Dosing Protocol for Increased Throughput in Label-Free Impedance-Based GPCR Assays

Published on: February 21, 2020

Label-free cell-based assays for GPCR screening.

Ye Fang1, Anthony G Frutos, Ronald Verklereen

  • 1Biochemical Technologies, Science and Technology Division, Corning Incorporated, Corning, NY 14831, USA. fangy2@corning.com

Combinatorial Chemistry & High Throughput Screening
|June 10, 2008
PubMed
Summary
This summary is machine-generated.

Label-free cell assay technologies are advancing rapidly for screening G protein-coupled receptors (GPCRs), the largest family of druggable targets. These biosensor-centered methods offer significant potential for future GPCR drug discovery programs.

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Area of Science:

  • Pharmacology
  • Biotechnology
  • Molecular Biology

Background:

  • G protein-coupled receptors (GPCRs) represent the largest family of druggable targets within the human genome.
  • The continuous de-orphanization of novel GPCR targets ensures their sustained importance in drug discovery pipelines.
  • Recent technological advancements in instrumentation and cellular signal measurement have spurred innovation in GPCR screening.

Purpose of the Study:

  • To review the fundamental principles of current label-free cell assay technologies.
  • To explore the potential applications of these technologies in GPCR drug discovery.
  • To highlight the significance of biosensor-centered assays for high-throughput GPCR screening.

Main Methods:

  • Review of existing literature on label-free cell assay technologies.
  • Analysis of biosensor principles and their application to GPCR signaling.
  • Discussion of instrumentation advancements enabling sensitive cellular measurements.

Main Results:

  • Label-free cell assay technologies are emerging as a powerful tool for GPCR screening.
  • These methods provide real-time, quantitative measurements of cellular responses without the need for exogenous labels.
  • Biosensor-centered approaches offer high sensitivity and throughput for identifying novel GPCR-modulating compounds.

Conclusions:

  • Label-free cell assay technologies hold significant promise for accelerating GPCR drug discovery.
  • The continued development of these technologies will be crucial for identifying new therapeutics targeting GPCRs.
  • The integration of biosensors with cell-based assays represents a key advancement in modern drug screening platforms.