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Updated: Jul 4, 2026
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Identification of Kinase-substrate Pairs Using High Throughput Screening
Published on: August 29, 2015
Current in vitro kinase assay technologies: the quest for a universal format.
Yong Jia1, Christopher M Quinn, Silvia Kwak
1Abbott Laboratories, 100 Research Drive, Worcester, MA 01605, USA. yong.jia@abbott.com
Current Drug Discovery Technologies
|June 10, 2008
Summary
Kinase assay technologies are crucial for drug discovery and fall into three main types. While generic assays show promise, no single technology is universally applicable yet.
Area of Science:
- Biochemistry
- Pharmacology
- Drug Discovery
Background:
- Kinases are key targets in drug development, driving innovation in assay technologies.
- Existing kinase assay technologies include radiometric, phospho-antibody-dependent, and phospho-antibody-independent methods.
Purpose of the Study:
- To review major kinase assay technologies, focusing on recent advancements.
- To detail the principles, pros, cons, and applications of these assays in drug discovery.
Main Methods:
- Categorization of kinase assay technologies into three main groups.
- Review of physical principles, practical aspects, and applications of various systems.
- Emphasis on newest systems and their suitability for high-throughput screening (HTS) and kinetic studies.
Main Results:
- Most current technologies are suitable for HTS, but few support kinetic and mechanistic studies.
- Progress has been made in developing generic assays, reducing reliance on radioisotopes and custom reagents.
- No single generic assay technology is universally applicable due to inherent limitations.
Conclusions:
- Selection of a kinase assay technology requires careful consideration of intended applications, cost, timeline, and expertise.
- While generic assays are advancing, a truly universal kinase assay remains an ongoing development goal.
