Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

The Physiology of Taste

The Physiology of Taste

The perception of a salty flavor is facilitated by sodium ions within the oral salivary fluid. Upon consumption of a salty substance, salt crystals disassemble, leading to the liberation of its constituents—Na+ and Cl- ions. These ions subsequently dissolve into the salivary fluid present in the oral cavity. The external environment of the gustatory cells experiences an elevation in Na+ concentration, thereby establishing a potent concentration gradient. This gradient propels the diffusion of...

Enhanced Elimination of Poison

Enhanced Elimination of Poison

Poison can be effectively removed from the gastrointestinal (GI) tract through various decontamination procedures.
Antidotes serve a crucial role in counteracting the effects of poison by inhibiting enzymes responsible for producing harmful drug metabolites. In some cases, these toxic metabolites can be neutralized by endogenous cosubstrates, which are maintained at specific concentrations to prevent interaction with cellular macromolecules and subsequent cell death.
Renal excretion is the...

Punishment

Punishment

Negative reinforcement and punishment are often confused but serve distinct functions in behavior modification. Reinforcement, whether positive or negative, increases the likelihood of a desired behavior, while punishment decreases it.
Punishment can be positive or negative. Positive punishment involves adding an undesirable stimulus, such as scolding, to decrease a behavior. Negative punishment involves removing a desirable stimulus, such as taking away a favorite toy, to decrease behavior.

Jealousy in Romantic Relationships

Jealousy in Romantic Relationships

Jealousy is an instinctive yet socially complex emotion that arises when a close relationship is threatened. Its origins lie in both biological imperatives and sociocultural conditioning, making it a multifaceted psychological construct. Although universally experienced, the triggers and expressions of jealousy vary notably between individuals, especially across genders, due to evolutionary pressures and cultural influences.Gender Differences and Evolutionary TheoryEvolutionary theory explains...

Conditioned Taste Aversion

Conditioned Taste Aversion

Conditioned taste aversion, also known as sauce béarnaise syndrome, is a phenomenon in which an individual develops an aversion to a certain food taste following a negative experience, typically illness. This form of aversion is a type of classical conditioning in which the taste of the food (conditioned stimulus, CS) is associated with the experience of illness (unconditioned stimulus, UCS).
A notable characteristic of conditioned taste aversion is that it often requires only a single exposure...

Competition

Competition

When organisms require the same limited resources within an environment, they may have to compete for them. Competition is a net-negative interaction. Even if two competing individuals or populations do not interact directly, the overall fitness of both competitors is lowered as a result of not having full access to the limited resource.Intraspecific competition, which occurs between individuals of the same species, serves as a natural mechanism for regulating population size. Too much...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Sensing centrosome amplification: the interface between centriole duplication and autophagy.

Nature communications·2026

Same author

Spindle-localized F-actin regulates polar MTOC organization and the fidelity of meiotic spindle formation.

Nature communications·2025

Same author

Spindle-localized F-actin regulates polar MTOC organization and the fidelity of meiotic spindle formation.

bioRxiv : the preprint server for biology·2025

Same author

Interactions of N- and C-terminal parts of Ana1 permitting centriole duplication but not elongation.

Open biology·2025

Same author

Interactions of N- and C-terminal parts of Ana1 permitting centriole duplication but not elongation.

bioRxiv : the preprint server for biology·2024

Same author

Greatwall-Endos-PP2A/B55<sup>Twins</sup> network regulates translation and stability of maternal transcripts in the <i>Drosophila</i> oocyte-to-embryo transition.

Open biology·2024

Search research articles

Related Experiment Video

Updated: Jul 4, 2026

Denver Papillae Protocol for Objective Analysis of Fungiform Papillae

Denver Papillae Protocol for Objective Analysis of Fungiform Papillae

Published on: June 8, 2015

A bitter PP1 fights the sweet polo.

Vincent Archambault¹, Feng Chen, David M Glover

¹Department of Genetics, Cancer Research UK Cell Cycle Genetics Research Group, University of Cambridge, Downing Street, CB2 3EH Cambridge, UK. va228@cam.ac.uk

|June 10, 2008

Summary

The protein MYPT1 interacts with PLK1, a key regulator of cell division. MYPT1 antagonizes PLK1

Area of Science:

Cell biology
Molecular biology
Biochemistry

Background:

Polo-like kinase 1 (PLK1) is crucial for cell division.
Regulatory subunits modulate kinase activity and function.

Purpose of the Study:

To investigate the interaction between MYPT1 and PLK1.
To determine how MYPT1 affects PLK1's mitotic functions.

Main Methods:

Co-immunoprecipitation assays to detect protein interactions.
In vitro kinase assays to assess dephosphorylation activity.

Main Results:

MYPT1 directly interacts with PLK1.
MYPT1 antagonizes PLK1's essential mitotic roles.
MYPT1 promotes PLK1 dephosphorylation at Thr210, reducing its activity.

More Related Videos

A Bilingual Computational Workflow for Identifying Potential PLK1 Inhibitors in American Sign Language and English

A Bilingual Computational Workflow for Identifying Potential PLK1 Inhibitors in American Sign Language and English

Published on: April 3, 2026

Taste Exam: A Brief and Validated Test

Taste Exam: A Brief and Validated Test

Published on: August 17, 2018

Related Experiment Videos

Last Updated: Jul 4, 2026

Denver Papillae Protocol for Objective Analysis of Fungiform Papillae

Denver Papillae Protocol for Objective Analysis of Fungiform Papillae

Published on: June 8, 2015

A Bilingual Computational Workflow for Identifying Potential PLK1 Inhibitors in American Sign Language and English

A Bilingual Computational Workflow for Identifying Potential PLK1 Inhibitors in American Sign Language and English

Published on: April 3, 2026

Taste Exam: A Brief and Validated Test

Taste Exam: A Brief and Validated Test

Published on: August 17, 2018

Conclusions:

MYPT1 acts as a negative regulator of PLK1 during mitosis.
The interaction and dephosphorylation mechanism highlight a novel regulatory pathway for PLK1.