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Arsenate reduces copper phytotoxicity in gametophytes of Pteris vittata
Yongqiang Zheng1, Xiaojing Dai, Lei Wang
1Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, PR China.
Abstract:
The fern Pteris vittata is an arsenic (As) hyperaccumulator and can take up very high concentrations of arsenic from the soil. However, little is known about its response to co-contamination with arsenic and copper (Cu). In this study, we used an in vitro model system of P. vittata gametophytes to investigate the impact of changes in As and Cu status on growth, chlorophyll (chl) concentration, metal accumulation, and subcellular localization. A remarkable inhibition of growth occurred when gametophytes were exposed to concentrations >or=1.0mM Na(3)AsO(4) or >or=0.5mM CuSO(4). chl concentration decreased significantly when gametophytes were exposed to >0.25mM of CuSO(4), but increased steadily with concentration to
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