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Related Concept Videos

Potentiometry: Membrane Electrodes01:15

Potentiometry: Membrane Electrodes

Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at the...
Mechanically-gated Ion Channels01:12

Mechanically-gated Ion Channels

Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
Mechanically-gated Ion Channels01:12

Mechanically-gated Ion Channels

Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
Patch Clamp01:18

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...
Ligand-gated Ion Channels01:19

Ligand-gated Ion Channels

Ligand-gated ion channels are transmembrane proteins with a channel for ions to pass through and a binding site for a ligand. The channel opens only when a ligand attaches to the binding site.
Three Subfamilies of Ligand-gated Ion Channels
Ligand-gated ion channels fall into three subfamilies. The 'Cys-loop' includes the nicotinic acetylcholine receptors, γ-aminobutyric acid (GABA), glycine, and 5-hydroxytryptamine receptors. The second one is the 'Pore-loop' channels that include the...
Non-gated Ion Channels01:24

Non-gated Ion Channels

Ion channels are specialized proteins on the plasma membrane that allow charged ions to pass down their electrochemical gradient. Their main function is to maintain the membrane potential which is critical for cell viability. These channels are either gated or non-gated and can transport more than a thousand ions within milliseconds for the cellular event to occur.
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism.

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Related Experiment Video

Updated: Jul 4, 2026

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores
09:43

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

Published on: October 31, 2013

An ON/OFF biosensor based on blockade of ionic current passing through a solid-state nanopore.

Manola Moretti1, Enzo Di Fabrizio, Stefano Cabrini

  • 1Dipartimento di Biologia e Protezione delle Piante, Università di Udine, Udine, Italy.

Biosensors & Bioelectronics
|June 10, 2008
PubMed
Summary

This study introduces a novel single nanopore biosensor using DNA hybrids for stable ionic current blockades. Molecular recognition triggers pore opening, enabling sensitive detection for routine biosensing applications.

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Monitoring Protein Adsorption with Solid-state Nanopores
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High Resolution Physical Characterization of Single Metallic Nanoparticles

Published on: June 28, 2019

Related Experiment Videos

Last Updated: Jul 4, 2026

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores
09:43

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

Published on: October 31, 2013

Monitoring Protein Adsorption with Solid-state Nanopores
08:51

Monitoring Protein Adsorption with Solid-state Nanopores

Published on: December 2, 2011

High Resolution Physical Characterization of Single Metallic Nanoparticles
09:56

High Resolution Physical Characterization of Single Metallic Nanoparticles

Published on: June 28, 2019

Area of Science:

  • Nanotechnology
  • Biotechnology
  • Biosensing

Background:

  • Single nanopores are promising for biosensing, but detecting transient analyte translocations is challenging.
  • Current methods require complex setups, limiting routine biosensing applications.

Purpose of the Study:

  • To develop a novel biosensing concept using a single nanopore with stable ionic current blockades.
  • To demonstrate a DNA-hybrid-based system for molecular recognition detection.

Main Methods:

  • Utilized a silicon nitride membrane with a single nanopore.
  • Constructed a complex of magnetic bead/streptavidin/biotin-DNA1/DNA2-biotin/streptavidin/Quantumdot-cluster through the pore.
  • Monitored DNA strand displacement triggered by complementary oligonucleotides.

Main Results:

  • Achieved a stable blockade of ionic current through the nanopore using DNA hybrids.
  • Demonstrated that molecular recognition events (DNA strand displacement) cause pore opening and ionic current restoration.
  • Successfully detected specific oligonucleotide binding events.

Conclusions:

  • The novel nanopore biosensing concept offers a stable and sensitive detection method.
  • This approach overcomes limitations of transient event detection in traditional nanopore sensing.
  • The system shows significant potential for convenient and routine biosensing.