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Updated: Jul 4, 2026

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

A nanogram-level colloidal gold single reagent quantitative protein assay.

Gerald Harrison¹, Patrick Haffey, Ellis E Golub

¹Department of Biochemistry, University of Pennsylvania School of Dental Medicine, Philadelphia, PA 19104, USA.

Analytical Biochemistry

|June 10, 2008

Summary

A new Golddots assay quantifies proteins at nanogram levels using colloidal gold and nitrocellulose paper. This method requires minimal sample volume and offers a sensitive alternative to traditional protein assays.

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Area of Science:

Biochemistry
Analytical Chemistry
Materials Science

Background:

Accurate protein quantification is crucial in biological research.
Existing protein assays often require larger sample volumes and more complex procedures.
There is a need for sensitive, rapid, and low-volume protein detection methods.

Purpose of the Study:

To develop and validate a novel nanogram-level quantitative protein assay.
To evaluate the performance of the Golddots assay compared to conventional methods.
To assess the assay's applicability to proteins with diverse physical properties.

Main Methods:

Developed a protein assay utilizing colloidal gold binding to proteins on nitrocellulose paper.
Quantified protein-gold complexes via densitometry of the resulting purple color.

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Calibrated the assay using weighed protein standards and compared it with the Pierce Micro BCA Protein Assay Kit.

Main Results:

The Golddots assay demonstrates nanogram-level sensitivity for protein quantification.
The assay requires minimal sample volume (2 mul) and protein amounts (1-5 mug).
Results showed good correlation with weighed protein samples and the Micro BCA assay.

Conclusions:

The Golddots assay provides a sensitive and efficient method for protein quantification.
Its low sample requirement makes it suitable for precious or limited biological samples.
This assay offers a valuable alternative for researchers needing rapid, high-sensitivity protein analysis.