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Related Concept Videos

High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For example, the mass of helium...
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
Mass Analyzers: Overview01:13

Mass Analyzers: Overview

The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
Mass Spectrum: Interpretation01:24

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An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a soft-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.To...

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Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
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Molecular mass concentrations for a powdered SRM sample using a quantitative single particle analysis.

M S I Khan1, H Hwang, H Kim

  • 1Department of Chemistry, Inha University, Incheon 402-751, Republic of Korea.

Analytica Chimica Acta
|June 10, 2008
PubMed
Summary

Low-Z particle electron probe microanalysis (EPMA) can now provide accurate mass concentration data for airborne aerosols. This quantitative single particle analysis complements bulk methods for better understanding aerosol behavior.

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Area of Science:

  • Environmental Science
  • Analytical Chemistry
  • Materials Science

Background:

  • Quantitative single particle analysis, low-Z particle electron probe microanalysis (EPMA), provides number concentration data for aerosol chemical species.
  • Mass concentration data from single particle analysis would enhance understanding of airborne aerosol behavior when combined with bulk analysis data.

Purpose of the Study:

  • To investigate the reliability of obtaining mass concentration data using the low-Z particle EPMA technique.
  • To demonstrate the utility of low-Z particle EPMA for quantitative single particle analysis.

Main Methods:

  • Analysis of a potassium feldspar powdered standard reference material (SRM) using the low-Z particle EPMA technique.
  • Comparison of elemental weight fractions obtained by low-Z particle EPMA with certified values from bulk analytical techniques.

Main Results:

  • Weight fractions of major elements determined by low-Z particle EPMA were within 8% of certified values from bulk techniques.
  • Low-Z particle EPMA successfully provided molecular mass concentration data for chemical species, which is challenging for bulk analysis.

Conclusions:

  • The low-Z particle EPMA technique reliably provides quantitative mass concentration data for single aerosol particles.
  • This method complements traditional bulk analysis, offering deeper insights into aerosol composition and behavior.