Related Experiment Video
Updated: Jul 4, 2026

Selection of Transporter-Targeted Inhibitory Nanobodies by Solid-Supported-Membrane (SSM)-Based Electrophysiology
Published on: May 3, 2021
New substrates for TonB-dependent transport: do we only see the 'tip of the iceberg'?
Kristine Schauer1, Dmitry A Rodionov, Hilde de Reuse
1Institut Pasteur, Unité Postulante de Pathogenèse de Helicobacter, Département de Microbiologie, 75724 Paris Cedex 15, France. Kristine.Schauer@curie.fr
Abstract:
TonB-dependent transport is a mechanism for active uptake across the outer membrane of Gram-negative bacteria. The system promotes transport of rare nutrients and was thought to be restricted to iron complexes and vitamin B12. Recent experimental evidence of TonB-energized transport of nickel and different carbohydrates, in addition to bioinformatic-based predictions, challenges this notion and reveals that the number and variety of TonB-dependent substrates is underestimated. It is becoming clear that the chemical nature of the substrates, the energetic requirements for transport and the subsequent translocation across the cytoplasmic membrane can differ from those of the well-studied systems for iron complexes and vitamin B12. These findings question the understanding of TonB-dependent uptake and provide insights into the adaptation of bacteria to their environments.
More Related Videos
11:05Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network Using Functionalized Nanobodies in Cultured Cells
Published on: February 21, 2019
08:55Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence (TIRF) Microscopy
Published on: February 17, 2023
Related Concept Videos
Secondary Active Transport
Secondary Active Transport
Secondary Active Transport
ABC Transporters: Exporter
Protein Transport to the Thylakoids
The Significance of Membrane Transport
Transporters facilitate either an active or passive movement of solutes. They can allow a single-molecule transport down its...