Purification, crystallization and preliminary crystallographic analysis of biotin protein ligase from Staphylococcus
Nicole R Pendini1, Steve W Polyak, Grant W Booker
1School of Molecular and Biomedical Sciences, University of Adelaide, North Terrace, Adelaide SA 5005, Australia.
Abstract:
Biotin protein ligase from Staphylococcus aureus catalyses the biotinylation of acetyl-CoA carboxylase and pyruvate carboxylase. Recombinant biotin protein ligase from S. aureus has been cloned, expressed and purified. Crystals were grown using the hanging-drop vapour-diffusion method using PEG 8000 as the precipitant at 295 K. X-ray diffraction data were collected to 2.3 A resolution from crystals using synchrotron X-ray radiation at 100 K. The diffraction was consistent with the tetragonal space group P4(2)2(1)2, with unit-cell parameters a = b = 93.665, c = 131.95.
