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Simian virus 40 mutants carrying two temperature-sensitive mutations.

J Y Chou

    Journal of Virology
    |October 1, 1976
    PubMed
    Summary
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    Simian virus 40 (SV40) double mutants reveal that the A complementation group is essential for viral DNA replication and host DNA synthesis. The A mutation

    Area of Science:

    • Virology
    • Molecular Biology
    • Genetics

    Background:

    • Simian virus 40 (SV40) is a valuable model for studying viral replication and DNA synthesis.
    • Temperature-sensitive mutants are crucial tools for dissecting viral gene function.

    Purpose of the Study:

    • To characterize the function of SV40 complementation groups, particularly group A, using double mutants.
    • To investigate the roles of specific SV40 genes in viral DNA replication, host DNA synthesis induction, and cell transformation.

    Main Methods:

    • Isolation and characterization of five temperature-sensitive SV40 mutants with two temperature-sensitive mutations.
    • Complementation analysis to assess the functional relationships between different SV40 mutants.
    • Assessing viral DNA replication, host DNA synthesis induction, and cell transformation at permissive and restrictive temperatures.

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    Main Results:

    • The SV40 A:D double mutant exhibited only the A defect under conditions suppressing the D defect, indicating A's essential role.
    • Viral DNA replication and host DNA synthesis were severely impaired in A:D double mutants at restrictive temperatures.
    • Double mutants involving complementation group A (A:B, A:C, A:BC) were defective in viral DNA replication, host DNA synthesis induction, and cell transformation.

    Conclusions:

    • Complementation group A is critical for SV40 viral DNA replication and the induction of host DNA synthesis.
    • The A mutation significantly impacts SV40's ability to transform cells.
    • SV40 double mutants provide insights into the complex genetic interactions governing viral functions.