Megakaryocytes and platelets in experimentally induced renovascular hypertension (2K1C) in rats

Hypertension is one of the most frequently occurring diseases worldwide. Approximately 10% of the population with hypertension reveals the secondary type of the disease. The aim of the study was to evaluate the megakaryocyte-platelet system in the course of renovascular hypertension.

An experimental model of hypertension in rats according to Goldblatt was used in the study. The experimental material (blood, bone marrow) was collected in the 4th, 8th, and 16th weeks of the study. Bone marrow megakaryocytes (MKs) were evaluated using immunohistochemical and morphometric methods. Blood platelets were analyzed based on their count (PLT) and mean volume (MPV). Plasma thrombopoietin (TPO) concentration was also assessed.

The investigation showed increased numbers of MKs 16 weeks after partial unilateral ligation of the renal artery. Statistically significant increase in platelet count, platelet mass, and the number of MK naked nuclei (NKs) as well as elevation of the circular deviation of the nuclei (CDN) of MKs accompanied the changes. MPV and TPO concentration did not change during the experiment. There was significant positive correlation between the increase in blood pressure and the numbers of MKs and NKs. The number of MKs correlated positively with PLT and CDN. Although TPO plasma level did not change significantly, there was marked negative correlation between plasma TPO concentration and PLT.

Although features of intensified platelet turnover were not observed, on the basis of the study it can be assumed that the megakaryocytic system undergoes changes in the course of renovascular hypertension. This can contribute to blood platelet production and the development of possible hypertension complications.