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Mouse strain resistant to N-, B-, and NB-tropic murine leukemia viruses
Abstract:
Mouse strain G was studied for its susceptibility to various strains of murine leukemia and sarcoma viruses. Both N- and NB-tropic Friend leukemia viruses neither induced splenomegaly nor grew efficiently in strain G mice. Using the XC test, cultured embryo cells were found to be resistant, but not absolutely, to all the tested viruses, N-tropic AKR virus, N- and NB-tropic Friend leukemia viruses, NB-tropic Rauscher leukemia virus, B-tropic WN1802B virus, NB-tropic Moloney leukemia and sarcoma viruses, and N-tropic Kirsten sarcoma virus, although the resistance to Moloney leukemia and sarcoma viruses is sometimes not as strong as that for other viruses. Thus, the strain G mice are unique among mouse strains because they show resistance that is not related to the N-B tropism of murine leukemia viruses.
Insights
Strain G mice exhibit unique resistance to multiple murine leukemia and sarcoma viruses. This resistance is independent of the N-B tropism typically associated with these viruses.
Area of Science:
- Virology
- Immunology
- Genetics
Background:
- Murine leukemia and sarcoma viruses (MLVs/MSVs) are significant pathogens in mouse models.
- Understanding host resistance mechanisms is crucial for viral pathogenesis research.
- Mouse strain genetics play a key role in viral susceptibility.
Purpose of the Study:
- To investigate the susceptibility of mouse strain G to various MLVs and MSVs.
- To characterize the resistance phenotype of strain G mice at the cellular level.
- To determine if strain G resistance is linked to known viral tropism patterns.
Main Methods:
- In vivo studies of splenomegaly and viral growth in strain G mice.
- In vitro XC plaque assays using cultured embryo cells from strain G.
- Testing against a panel of N-tropic, NB-tropic, and B-tropic MLVs and MSVs.
Main Results:
- Strain G mice showed resistance to N- and NB-tropic Friend leukemia viruses, with no splenomegaly or efficient viral growth.
- Cultured embryo cells from strain G were resistant to all tested viruses, including N-tropic AKR, Friend, Rauscher, Moloney, and Kirsten viruses.
- Resistance was observed against N-, NB-, and B-tropic viral strains, indicating a broad-spectrum resistance.
Conclusions:
- Mouse strain G possesses a unique resistance mechanism against a wide range of MLVs and MSVs.
- This resistance is not determined by the N-B tropism of the viruses, differentiating it from other known resistance mechanisms.
- Strain G mice represent a novel model for studying host antiviral immunity independent of viral tropism.