Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Streamlining Diagnosis of Bardet-Biedl Syndrome: New Diagnostic Algorithm With Updated Criteria.

American journal of medical genetics. Part A·2026
Same author

Endocrine Characteristics in Bardet Biedl Syndrome from a Large Single-Centre Paediatric Multidisciplinary Clinic.

The Journal of clinical endocrinology and metabolism·2026
Same author

Quantitative Perspectives of Biomolecular Crystallization Thermodynamics.

Advances in biochemical engineering/biotechnology·2026
Same author

Impact of Setmelanotide on Metabolic Syndrome Risk in Patients With Bardet-Biedl Syndrome.

The Journal of clinical endocrinology and metabolism·2025
Same author

Burden of hyperphagia and obesity in Bardet-Biedl syndrome: a multicountry survey.

Orphanet journal of rare diseases·2023
Same author

Caregiver burden in Bardet-Biedl syndrome: findings from the CARE-BBS study.

Orphanet journal of rare diseases·2023

Related Experiment Video

Updated: Jul 4, 2026

Optimizing the Growth of Endothiapepsin Crystals for Serial Crystallography Experiments
09:52

Optimizing the Growth of Endothiapepsin Crystals for Serial Crystallography Experiments

Published on: February 4, 2021

Fluorescence approaches to growing macromolecule crystals.

Marc Pusey1, Elizabeth Forsythe, Aniruddha Achari

  • 1MI Research Inc, Huntsville, AL, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 11, 2008
PubMed
Summary

Trace fluorescent labeling helps identify macromolecule crystallization by detecting bright spots in precipitates. This method simplifies distinguishing crystals from amorphous material, aiding in discovering crystallization conditions.

More Related Videos

Optimization of Crystal Growth for Neutron Macromolecular Crystallography
12:29

Optimization of Crystal Growth for Neutron Macromolecular Crystallography

Published on: March 13, 2021

Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect
06:42

Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect

Published on: March 19, 2019

Related Experiment Videos

Last Updated: Jul 4, 2026

Optimizing the Growth of Endothiapepsin Crystals for Serial Crystallography Experiments
09:52

Optimizing the Growth of Endothiapepsin Crystals for Serial Crystallography Experiments

Published on: February 4, 2021

Optimization of Crystal Growth for Neutron Macromolecular Crystallography
12:29

Optimization of Crystal Growth for Neutron Macromolecular Crystallography

Published on: March 13, 2021

Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect
06:42

Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect

Published on: March 19, 2019

Area of Science:

  • Biochemistry
  • Crystallography
  • Biophysics

Background:

  • Macromolecule crystallization is crucial for structural determination.
  • Trace fluorescent labeling is a potential tool to monitor crystallization.
  • Distinguishing crystalline from amorphous precipitates can be challenging.

Purpose of the Study:

  • To evaluate trace fluorescent labeling as an indicator of macromolecule crystallization.
  • To determine if fluorescence intensity can predict crystallization success.
  • To explore using "bright spots" as indicators for crystallization conditions.

Main Methods:

  • Utilizing trace fluorescent labeling (less than 1%) in macromolecule precipitation experiments.
  • Observing fluorescence intensity of solid phases under various conditions.
  • Analyzing "bright spots" in precipitates not visible under white light.

Main Results:

  • Higher fluorescence intensity correlates with densely packed crystalline material.
  • Fluorescence allows clear distinction between crystalline and amorphous phases.
  • "Bright spots" observed via fluorescence indicate potential crystallization conditions.

Conclusions:

  • Trace fluorescent labeling is an effective method for monitoring macromolecule crystallization.
  • Fluorescence intensity serves as a reliable indicator of crystal formation.
  • The presence of "bright spots" can guide the discovery of novel crystallization conditions.