Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

X-ray Crystallography

X-ray Crystallography

The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Author Correction: Sequence specificity analysis of the SETD2 protein lysine methyltransferase and discovery of a SETD2 super-substrate.

Communications biology·2020

Same author

Sequence specificity analysis of the SETD2 protein lysine methyltransferase and discovery of a SETD2 super-substrate.

Communications biology·2020

Same author

Data on post bank customer reviews from web.

Data in brief·2020

Same author

Structural Basis for the Binding Selectivity of Human CDY Chromodomains.

Cell chemical biology·2020

Same author

Structural Basis for EPC1-Mediated Recruitment of MBTD1 into the NuA4/TIP60 Acetyltransferase Complex.

Cell reports·2020

Same author

Discovery of Small Molecule Antagonists of the USP5 Zinc Finger Ubiquitin-Binding Domain.

Journal of medicinal chemistry·2019

Search research articles

Related Experiment Video

Updated: Jul 4, 2026

High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

Published on: March 10, 2023

High throughput crystallography at SGC Toronto: an overview.

Alexey Bochkarev¹, Wolfram Tempel

¹Structural Genomics Consortium, University of Toronto, Toronto, ON, Canada.

Methods in Molecular Biology (Clifton, N.J.)

|June 11, 2008

Summary

This summary is machine-generated.

The Structural Genomics Consortium (SGC) analyzes human protein families to develop therapeutic reagents. Their family-based approach and high-throughput methods offer a model for structural biology laboratories.

More Related Videos

Fully Autonomous Characterization and Data Collection from Crystals of Biological Macromolecules

Fully Autonomous Characterization and Data Collection from Crystals of Biological Macromolecules

Published on: March 22, 2019

Workflow and Tools for Crystallographic Fragment Screening at the Helmholtz-Zentrum Berlin

Workflow and Tools for Crystallographic Fragment Screening at the Helmholtz-Zentrum Berlin

Published on: March 3, 2021

Related Experiment Videos

Last Updated: Jul 4, 2026

High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

Published on: March 10, 2023

Fully Autonomous Characterization and Data Collection from Crystals of Biological Macromolecules

Fully Autonomous Characterization and Data Collection from Crystals of Biological Macromolecules

Published on: March 22, 2019

Workflow and Tools for Crystallographic Fragment Screening at the Helmholtz-Zentrum Berlin

Workflow and Tools for Crystallographic Fragment Screening at the Helmholtz-Zentrum Berlin

Published on: March 3, 2021

Area of Science:

Structural biology
Genomics
Biochemistry

Background:

The completion of the human genome enables analysis of biological systems through entire gene families.
Enzyme studies can explore complex substrate specificity networks with overlapping specificities within and between protein families.

Purpose of the Study:

To determine the structures of proteins relevant to human health.
To place protein structures into the public domain without restrictions.
To develop a family-based approach for protein studies, particularly for therapeutic reagent development.

Main Methods:

Crystallography methods tailored for human protein families.
Increased reliance on in-house X-ray sources for diffraction data collection.
Predominant use of molecular replacement for phase determination.
High-throughput (HTP) sample and data flow management.

Main Results:

The SGC's approach differs from structural genomics projects focused on protein fold space.
The SGC's methods are becoming an increasingly useful model for structural biology laboratories.
Technical details of HTP workflow at SGC Toronto are presented.

Conclusions:

A family-based approach to protein studies is compelling for exploiting specificities.
The SGC's methodology provides a paradigm for collaborative or shared X-ray instrumentation facilities.
The SGC's work contributes significantly to understanding human health-related proteins.