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Updated: Jul 4, 2026
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The MODS method for diagnosis of tuberculosis and multidrug resistant tuberculosis
Published on: August 11, 2008
Programmatic implementation of rapid DST for Mycobacterium tuberculosis in Peru
1Instituto Nacional de Salud, Lima, Peru.
Summary
A rapid drug susceptibility test (DST) for Mycobacterium tuberculosis, the Griess method, significantly reduced testing time from 99 to 31 days compared to conventional DST. This demonstrates favorable performance for tuberculosis diagnostics in public health settings.
Area of Science:
- Clinical microbiology
- Infectious disease diagnostics
- Public health
Background:
- Rapid drug susceptibility testing (DST) for Mycobacterium tuberculosis requires evaluation in real-world public health settings.
- Performance of novel diagnostic methods can differ between research and implementation.
Purpose of the Study:
- To evaluate the performance characteristics of a direct, rapid DST for Mycobacterium tuberculosis in Lima, Peru.
- To compare the efficiency and accuracy of the Griess method against conventional DST.
Main Methods:
- A district laboratory validated both conventional DST and the nitrate reductase (Griess) method.
- Data on 4102 conventional DSTs and 895 Griess tests from January 2005 to June 2007 were analyzed.
- Frequency of testing, results, and median time to result were calculated.
Main Results:
- The Griess method yielded results for 70.2% of samples, compared to 72.8% for conventional DST.
- Multidrug-resistant tuberculosis prevalence was 31.5% with Griess and 26.4% with conventional DST.
- Median time to result was significantly reduced with Griess (31 days) versus conventional DST (99 days).
Conclusions:
- The Griess method shows promising performance characteristics under actual public health program conditions.
- This rapid DST offers a substantial reduction in turnaround time for tuberculosis diagnosis.
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