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Updated: Jul 4, 2026

An Automated Culture System for Use in Preclinical Testing of Host-Directed Therapies for Tuberculosis
Published on: August 16, 2021
Tuberculosis culture testing at a tertiary care hospital: options for improved management and use for treatment
M E Edginton1, L Rakgokong, S Verver
1School of Public Health, University of the Witwatersrand, Johannesburg, South Africa. maryhodkinson@gmail.com
Setting:
Chris Hani Baragwanath Hospital, a tertiary care hospital in Johannesburg, South Africa.
Objectives:
To determine the proportion of patients with positive Mycobacterium tuberculosis cultures and whether those who were positive were on treatment.
Design:
Tuberculosis (TB) culture results were obtained from the laboratory for a 3-month period. Positive results were checked against registrations at the hospital TB Care Centre (TBCC). The treatment status of non-registered patients was obtained from various records at the hospital, district clinics and from home visits.
Results:
Overall, 3909 patients had 5404 samples sent for culture. Of these, 708 patients (18%) had at least one positive culture. The positive yield from 2749 adult sputum samples was 33% and ranged from 6% to 40% for different extra-pulmonary specimens. Among 1160 children, the yield varied from 0% to 12%, with 12% in sputum and gastric washing specimens. Of the 708 culture-positive patients, 429 (61%) patients were registered at the TBCC and were known to have started TB treatment. Of the 279 subjects not registered (39% overall), 100 (36%) died. Only 67 of the 179 survivors were confirmed on treatment, 40 were not on treatment and 72 could not be traced.
Conclusions:
Large numbers of TB culture tests were performed, some inappropriately. Study findings highlight inadequacies in the management of culture-confirmed TB at this hospital.
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