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Anaphase Promoting Complex

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...

Anaphase Promoting Complex

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...

Separation of Sister Chromatids

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...

The Cell Cycle Control System

The Cell Cycle Control System

The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...

The Cell Cycle Control System

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...

The Cell Cycle Control System

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...

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Related Experiment Video

Updated: Jul 4, 2026

Analysis of Cell Cycle Position in Mammalian Cells

Analysis of Cell Cycle Position in Mammalian Cells

Published on: January 21, 2012

To cell cycle, swing the APC/C.

Renske van Leuken¹, Linda Clijsters, Rob Wolthuis

¹Department of Medical Oncology, University Medical Center, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands.

Biochimica Et Biophysica Acta

|June 12, 2008

Summary

The Anaphase-Promoting Complex/Cyclosome (APC/C) regulates cell cycle progression by degrading key proteins. Understanding APC/C control is crucial for cancer treatment development.

Area of Science:

Cell Biology
Molecular Biology
Biochemistry

Background:

The Anaphase-Promoting Complex/Cyclosome (APC/C) is a crucial E3 ubiquitin ligase complex.
APC/C mediates the timely degradation of key cell cycle regulators, including Cyclin B1 and Securin, essential for mitotic progression.
APC/C substrates control diverse cellular events beyond mitosis, including G1 phase progression and maintaining quiescence.

Purpose of the Study:

To elucidate the regulatory mechanisms governing APC/C activity.
To highlight the broader roles of APC/C in cell cycle control and quiescence.
To underscore the therapeutic potential of targeting APC/C in cancer.

Main Methods:

Literature review and synthesis of existing research on APC/C function and regulation.

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Discussion of APC/C substrate identification and degradation pathways.

Analysis of APC/C interactions with cell cycle inhibitors and checkpoints.

Main Results:

APC/C activity is tightly regulated throughout the cell cycle, involving mechanisms that hold it in check or release it.
APC/C plays a critical role in maintaining cell cycle arrest in quiescent cells through stabilization of specific proteins.
The spindle assembly checkpoint is a key regulator of APC/C activity during prometaphase.

Conclusions:

The APC/C is a central regulator of cell cycle progression, with critical roles extending from mitosis to quiescence.
Dysregulation of APC/C activity is implicated in cancer development.
Targeting APC/C offers a promising avenue for novel cancer therapies.