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Updated: Jul 4, 2026

Performing In Vivo and Ex Vivo Electrical Impedance Myography in Rodents
Published on: June 8, 2022
Multivariate analysis of electrical impedance spectra for relaxed and contracted skeletal muscle
1University of Ljubljana, Faculty of Electrical Engineering, Ljubljana, Slovenia. tomaz.zagar@fe.uni-lj.si
Abstract:
Four-electrode impedance spectra of relaxed and contracted muscle biceps brachii were analyzed in an adult human subject over the frequency range from 300 Hz to 75 kHz. A feasibility of the principal component analysis of bioimpedance measurement for the evaluation of skeletal muscle contractile state was examined. The principal components score plots show a data grouping of the impedance spectra from the two muscle groups. The classification was performed using a soft independent modeling of class analogy (SIMCA) method. The data set comprised 32 samples (16 samples of contracted muscle and 16 samples of relaxed muscle). The leave-one-out test of the classification yields about 80% of correctly classified samples (11 samples for contracted and 15 samples for relaxed muscle).
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