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Related Experiment Video

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Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

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Fluorescence tomography characterization for sub-surface imaging with protoporphyrin IX.

Dax Kepshire¹, Scott C Davis, Hamid Dehghani

¹Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA. Dax@Dartmouth.edu

|June 12, 2008

Summary

This study compared fluorescence remittance imaging (FRI) and subsurface fluorescence diffuse optical tomography (FDOT) for optical imaging. Combining both methods offers optimal sensitivity and depth detection for localizing embedded fluorescent regions in tissue.

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Area of Science:

Biomedical Optics
Medical Imaging
Fluorescence Imaging

Background:

Non-contact optical imaging techniques are crucial for in-vivo diagnostics.
Characterizing subsurface fluorescent objects in tissue phantoms is essential for developing advanced imaging systems.

Purpose of the Study:

To evaluate and compare fluorescence remittance imaging (FRI) and subsurface fluorescence diffuse optical tomography (FDOT) for imaging embedded fluorescent objects.
To determine the optimal combination of imaging modalities for accurate localization of fluorescent regions within tissue.

Main Methods:

Experiments utilized tissue phantoms with varying contrast ratios (3.5:1 to 10:1) using Protoporphyrin IX as a fluorescent agent.
Non-contact approaches, including FRI and FDOT, were employed to image embedded fluorescent inclusions.
Performance was assessed based on contrast recovery and contrast-to-noise ratio.

Main Results:

FDOT successfully recovered interior inclusions with high contrast.
Fluorescence remittance imaging (FRI) demonstrated a superior contrast-to-noise ratio, particularly for surface-level objects.
FDOT provided crucial depth information for embedded regions.

Conclusions:

A combination of FRI and FDOT is proposed for optimal localization of embedded fluorescent regions.
Integrating FRI's sensitivity with FDOT's depth detection capabilities enhances accuracy in all three spatial dimensions within tissue.