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Detection of Clostridium perfringens type A enterotoxin in faecal and food samples using immunomagnetic separation
K S Cudjoe1, L I Thorsen, T Sørensen
1MATFORSK, Norwegian Food Research Institute, As.
International Journal of Food Microbiology
|April 1, 1991
Abstract:
A simple, rapid and sensitive immunoassay, based on immunomagnetic particles (Dynabeads M-280) was developed for detection and quantitation of Clostridium perfringens type A enterotoxin from faecal and food extracts. The assay had a detection limit of 2.5 ng/ml enterotoxin in homogenates of faeces and inoculated meat extracts. The specificity was confirmed by both crossed immunoelectrophoresis and Western immunoblotting techniques, using a purified enterotoxin as standard.