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Related Experiment Video

Updated: Jul 4, 2026

A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood
08:17

A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood

Published on: February 8, 2016

Antibody-bound cell microarray for immunophenotyping: surface modification and lymphocyte subpopulations.

Yasuo Fujii1, James M Anderson, Takehisa Matsuda

  • 1Department of Biomedical Engineering, Graduate School of Medicine, Kyushu University, Higashiku, Fukuoka 812-8582, Japan.

Journal of Biomedical Materials Research. Part B, Applied Biomaterials
|June 12, 2008
PubMed
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Antibody microarrays precisely quantify blood cell types for medical diagnosis. This high-throughput method offers reliable immunophenotyping using specific antibodies to cluster of differentiation (CD) antigens.

Area of Science:

  • Biomedical Engineering
  • Immunology
  • Materials Science

Background:

  • Antibody-based cell microarrays are crucial for high-throughput diagnostics.
  • Precise surface design is essential for biological specificity, reliability, and validity.
  • Quantitative immunophenotyping requires robust cell microarray platforms.

Purpose of the Study:

  • To quantitatively evaluate immunophenotyping using a cell microarray.
  • To assess the specificity and reliability of antibody fixation on microarrays.
  • To validate the accuracy of CD4/CD8 subset ratio determination.

Main Methods:

  • Fabrication of microarrays using photopatterned self-assembled monolayers of alkanethiols.
  • Covalent fixation of anti-CD4 and anti-CD8 antibodies onto carboxylated domains.

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A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood
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A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood

Published on: February 8, 2016

Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing
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Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing

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  • Verification of antibody fixation using confocal laser scanning microscopy and dye staining.
  • Validation of cell adhesion specificity using flow cytometry and magnetic bead isolation.

    • Main Results:

    • Microarrays demonstrated precise, high-specificity binding of peripheral blood mononuclear cells expressing CD4 or CD8 antigens.
    • Confocal microscopy confirmed near-complete antibody conversion during fixation.
    • CD4/CD8 subset ratios determined by microarrays closely matched flow cytometry results.
    • The microarray platform exhibited high biological specificity, reliability, and validity.

    Conclusions:

    • Covalently fixed antibodies on microarrays provide a foundation for high-throughput immunophenotyping.
    • This technology supports accurate quantitative evaluation for medical diagnosis.
    • The developed microarray technique offers a reliable tool for analyzing blood cell populations.