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Updated: Jul 4, 2026

Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes
Published on: January 16, 2016
Kinetics of immobilized sucrose phosphorylase
1Laboratory of Renewable Resources Engineering, A. A. Potter Engineering Center, Purdue University, West Lafayette, Indiana 47907, USA.
Abstract:
Sucrose phosphorylase was immobilized on porous ceramic beads with 3-aminopropyltriethoxysilane and glutaraldehyde. It was determined experimentally that under laboratory conditions there was no diffusional resistance to the enzyme-catalyzed reaction. The half-life of the immobilized enzyme varied from about 35 days at 30 degrees C to about 5 days at 40 degrees C. The pH optimum was found to be between 6.5 and 7.0. The activation energy for the reaction was found to be about 12.5 kcal/mol. Eleven independent kinetic constants in the complete rate equation for the previously proposed ping-pong mechanism were found to be in good agreement with those for the soluble enzyme.
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