Potentiometric quantitation of glycerol using immobilized glycerol dehydrogenase

Glycerol dehydrogenase was immobilized in polyacrylamide gel layered over a small platinum screen and used to catalyze the oxidation of glycerol. In the presence of NAD(+) and potassium ferricyanide, the coupling reaction generated a measurable electrical potential which was found to be Nernstian with respect to the glycerol concentration range of 10(-4)M to 10(-1)M. The reproducibility of the measurement and the optimal conditions for glycerol determination were described.