Related Experiment Video
Updated: Jul 4, 2026

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing
Published on: October 3, 2018
Comparative pair-wise domain-combinations for screening the clade specific domain-architectures in metazoan genomes
Shuichi Kawashima1, Takeshi Kawashima, Nicholas H Putnam
1Human Genome Center, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan. shuichi@hgc.jp
Abstract:
In the evolution of the eukaryotic genome, exon or domain shuffling has produced a variety of proteins. On the assumption that each fusion event between two independent protein-domains occurred only once in the evolution of metazoans, we can roughly estimate when the fusion events were happened. For this purpose, we made phylogenetic profiles of pair-wise domain-combinations of metazoans. The phylogenetic profiles can be expected to reflect the protein evolution of metazoan. Interestingly, the phylogenetic tree of metazoans, derived from the profiles, supported the "Ecdysozoa hypothesis" that is one of the major hypotheses for metazoan evolution. Further, the phylogenetic profiles showed the candidates of genes that were required for each clade-specific features in metazoan evolution. We propose that comparative proteome analysis focusing on pair-wise domain-combinations is a useful strategy for researching the metazoan evolution. Additionally, we found that the extant ecdysozoans share only fourteen domain-combinations in our profiles. Such a small number of ecdysozoan-specific domain-combinations is consistent with the extensive gene-losses through the evolution of ecdysozoans.
Related Concept Videos
Evolutionary Relationships through Genome Comparisons
Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes
Conservation of Protein Domains Over Different Proteins
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to form...
Conservation of Protein Domains
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to form...
Multi-species Conserved Sequences
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...
Three-Domain System of Life

