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Dialysis

Dialysis

Dialysis is a diffusion-based purification process that separates analyte molecules from a complex matrix. This is accomplished by allowing molecules in the solution to pass through a semipermeable membrane into a liquid on the other side. The membrane is usually made of cellulose acetate or cellulose nitrate, and the second liquid must be miscible with the solution. Ions (e.g., chloride or sodium) or organic molecules (e.g., glucose) can pass through the membrane pores, which generally have...

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Related Experiment Video

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Microdialysis of Excitatory Amino Acids During EEG Recordings in Freely Moving Rats

Microdialysis of Excitatory Amino Acids During EEG Recordings in Freely Moving Rats

Published on: November 8, 2018

Improved temporal resolution for in vivo microdialysis by using segmented flow.

Meng Wang¹, Gregory T Roman, Kristin Schultz

¹Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA.

Analytical Chemistry

|June 13, 2008

Summary

This summary is machine-generated.

This study introduces a microdialysis system using segmented flow for improved real-time monitoring of concentration dynamics. The novel approach enhances temporal resolution, crucial for in vivo experiments and glucose monitoring in rats.

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Last Updated: Jul 4, 2026

Microdialysis of Excitatory Amino Acids During EEG Recordings in Freely Moving Rats

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Published on: November 8, 2018

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In Vivo Microdialysis Method to Collect Large Extracellular Proteins from Brain Interstitial Fluid with High-molecular Weight Cut-off Probes

In Vivo Microdialysis Method to Collect Large Extracellular Proteins from Brain Interstitial Fluid with High-molecular Weight Cut-off Probes

Published on: September 26, 2018

Area of Science:

Analytical Chemistry
Biomedical Engineering
Neuroscience

Background:

Microdialysis is vital for in vivo monitoring but often lacks sufficient temporal resolution.
Taylor dispersion can degrade sample integrity and temporal resolution during transport.

Purpose of the Study:

To develop and validate a microdialysis system with enhanced temporal resolution using segmented flow.
To demonstrate the system's capability for real-time monitoring of analytes, including glucose.

Main Methods:

Interfacing microdialysis probes with a segmented flow system using a PDMS chip and immiscible carrier phase (perfluorodecalin).
Controlling plug volume, interval, and frequency by adjusting oil and sample flow rates.
Assessing temporal resolution via fluorescence measurements and demonstrating glucose detection using an enzyme assay.

Main Results:

Achieved temporal resolution as high as 15 seconds, independent of flow rate and transport distance, by preventing Taylor dispersion.
Demonstrated significantly improved temporal resolution compared to non-segmented flow systems.
Successfully measured in vivo glucose concentrations in anesthetized rats, showing a 60% decrease upon high-K+ stimulation.

Conclusions:

Segmented flow microdialysis offers superior temporal resolution for dynamic in vivo monitoring.
The developed system is suitable for real-time measurement of biologically relevant analytes like glucose.
This technology holds significant potential for advancing neuroscience and other in vivo research applications.