Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Insulin Secretory Vesicles01:05

Insulin Secretory Vesicles

Insulin secretory vesicles release insulin to stimulate blood glucose uptake and regulate carbohydrate metabolism. When the blood glucose levels increase, glucose enters the pancreatic β-islet cells through glucose transporters. Once inside, glucose is metabolized through glycolysis, the citric acid cycle, and the electron transport chain, producing ATP. This increase in ATP concentration closes ATP-sensitive potassium channels, leading to depolarization of the membrane and the opening of...
Glucose Homeostasis: Pancreatic Islets and Insulin Secretion01:27

Glucose Homeostasis: Pancreatic Islets and Insulin Secretion

The pancreatic islets comprising only 1%-2% of the volume are highly vascularized and innervated mini-organs. They contain five endocrine cell types, including β cells that secrete insulin, which is synthesized as a single polypeptide chain, preproinsulin, processed to proinsulin, and finally to insulin and C-peptide. This process is complex and regulated, involving the Golgi complex, the endoplasmic reticulum, and the secretory granules of the β cell.
Insulin and C-peptide are co-secreted in...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fibre morphology, intramyocellular lipid content and 3D capillary architecture in human postural, respiratory and locomotor muscles in type 2 diabetes mellitus.

Histochemistry and cell biology·2026
Same author

Sirolimus Versus Mycophenolate Mofetil in Simultaneous Pancreas-Kidney Transplantation: Impact on Urinary Tract Infection Rates.

Journal of transplantation·2026
Same author

ISL1 Restricts Progenitor Programs and Promotes β-Cell Maturation, Revealing Sex Differences in Diabetes Progression.

Diabetes·2026
Same author

Bone Benefits After Simultaneous Pancreas-kidney Transplantation Compared With the Pretransplant Period.

Transplantation·2026
Same author

In-vivo optical properties spectra across five body locations on ten subjects using time-domain diffuse optics.

Scientific data·2026
Same author

Characterization of diabetes-related biomechanical and structural alterations in human deep fasciae using atomic force microscopy and multimodal multiphoton imaging.

Tissue & cell·2025

Related Experiment Video

Updated: Jul 4, 2026

In situ Quantification of Pancreatic Beta-cell Mass in Mice
09:50

In situ Quantification of Pancreatic Beta-cell Mass in Mice

Published on: June 7, 2010

Imaging the Beta-cell mass: why and how.

Frantisek Saudek1, Carl-Henrik Brogren, Srirang Manohar

  • 1Diabetes Center, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 14021 Prague 4, Czech Republic.

The Review of Diabetic Studies : RDS
|June 13, 2008
PubMed
Summary

Accurately monitoring native beta-cell mass is crucial for understanding diabetes. The monoclonal antibody IC2 shows promise as a specific marker for noninvasive imaging of beta-cells, advancing diabetes research.

More Related Videos

Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research
15:18

Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research

Published on: January 12, 2013

Analysis of Beta-cell Function Using Single-cell Resolution Calcium Imaging in Zebrafish Islets
08:50

Analysis of Beta-cell Function Using Single-cell Resolution Calcium Imaging in Zebrafish Islets

Published on: July 3, 2018

Related Experiment Videos

Last Updated: Jul 4, 2026

In situ Quantification of Pancreatic Beta-cell Mass in Mice
09:50

In situ Quantification of Pancreatic Beta-cell Mass in Mice

Published on: June 7, 2010

Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research
15:18

Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research

Published on: January 12, 2013

Analysis of Beta-cell Function Using Single-cell Resolution Calcium Imaging in Zebrafish Islets
08:50

Analysis of Beta-cell Function Using Single-cell Resolution Calcium Imaging in Zebrafish Islets

Published on: July 3, 2018

Area of Science:

  • Endocrinology
  • Medical Imaging
  • Immunology

Background:

  • Diabetes mellitus is characterized by beta-cell loss and insulin deficiency, with limited understanding of its pathogenesis and therapeutic recovery strategies.
  • Current methods for monitoring beta-cell mass in vivo are insufficient, relying on indirect techniques or post-mortem analysis.
  • Existing in vivo imaging approaches for islet cells are limited to transplanted cells labeled with iron nanoparticles for MRI, not native beta-cells.

Purpose of the Study:

  • To identify a reliable beta-cell-specific marker for noninvasive in vivo imaging to advance the understanding of diabetes pathogenesis.
  • To evaluate potential imaging agents for their specificity and efficacy in detecting native beta-cell mass.
  • To explore new avenues for monitoring beta-cell mass under disease and therapeutic conditions.

Main Methods:

  • Investigated potential beta-cell-specific markers including ligands for vesicular monoamine transporter type 2 (VMAT-2), antibodies to zinc transporter 8 (ZnT-8), and the monoclonal antibody IC2.
  • Assessed the binding specificity of these candidates to beta-cells using various experimental models.
  • Utilized radioisotope-chelated IC2 for ex vivo imaging of pancreatic tissue to evaluate its detection capabilities.

Main Results:

  • Dihydrotetrabenazine (DTBZ) and ZnT-8 antibodies demonstrated binding to cells other than beta-cells.
  • The monoclonal antibody IC2 exhibited exclusive binding properties to insulin-producing beta-cells, confirmed by previous and recent investigations.
  • Ex vivo experiments showed specific binding of radioisotope-labeled IC2 to the beta-cell surface, detectable by nuclear imaging.

Conclusions:

  • The monoclonal antibody IC2 is currently the most promising marker for noninvasive functional imaging of native beta-cells.
  • This finding may lead to novel methods for monitoring beta-cell mass in vivo, crucial for diabetes research and treatment development.
  • Future research with IC2 could significantly enhance our understanding of diabetes pathogenesis and inform islet regeneration strategies.