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Adsorption of cellulase from Trichoderma viride on cellulose
H Ooshima1, M Sakata, Y Harano
1Department of Applied Chemistry, Faculty of Engineering, Osaka City University, Osaka 558, Japan.
Biotechnology and Bioengineering
|December 1, 1983
Summary
Cellulase enzyme adsorption on cellulose follows Langmuir isotherm. Temperature and cellulose crystallinity affect enzyme component adsorption, with beta-glucosidase adsorption being temperature-dependent.
Area of Science:
- Biochemistry
- Enzyme kinetics
- Materials science
Background:
- Cellulase enzymes are crucial for biomass degradation.
- Understanding cellulase adsorption on cellulose is key to optimizing enzymatic hydrolysis.
- Trichoderma viride cellulase (Meicelase CEP) is a widely studied enzyme complex.
Purpose of the Study:
- To investigate the adsorption characteristics of cellulase from Trichoderma viride on pure cellulose.
- To determine the influence of temperature and cellulose crystallinity on cellulase adsorption.
- To elucidate the adsorption behavior of individual cellulase components (endoglucanase and exoglucanase).
Main Methods:
- Studying the adsorption of Meicelase CEP on pure cellulose.
- Applying the Langmuir adsorption isotherm model.
- Analyzing the effects of temperature and cellulose crystallinity on adsorption parameters.
- Investigating the adsorption of CMCase (endoglucanase) and Avicelase (exoglucanase) components.
Main Results:
- Cellulase adsorption on pure cellulose follows the Langmuir isotherm.
- Temperature and cellulose crystallinity significantly impact Langmuir adsorption parameters.
- Endoglucanase and exoglucanase adsorption characteristics were elucidated.
- Beta-glucosidase adsorbed at 5°C but not at 50°C, indicating temperature-dependent adsorption.
Conclusions:
- Cellulase adsorption on cellulose is a complex process influenced by multiple factors.
- The Langmuir model provides a useful framework for describing cellulase adsorption.
- Differential adsorption of cellulase components and temperature sensitivity of beta-glucosidase are important considerations for industrial applications.
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