Related Experiment Video
Updated: Jul 4, 2026
12:11
ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection
Published on: October 23, 2009
Determination of substrate concentrations by a computerized enzyme electrode
J P Kernevez1, L Konate, J L Romette
1U.T.C., BP 233, 60206 Compiène, France.
Biotechnology and Bioengineering
|March 1, 1983
Summary
This study presents a novel numerical method for determining substrate concentrations, such as glucose, using artificial enzyme membranes and electrode signals. The approach accurately calculates glucose levels by analyzing transient electrode data and employing polynomial approximations for automated sample analysis.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Biosensors
Background:
- Enzyme electrodes offer a method for quantifying various substrates in solution.
- Accurate determination of substrate concentrations is crucial for diagnostics and research.
- Existing methods may require complex calibration or lack automation.
Purpose of the Study:
- To develop a numerical treatment for analyzing signals from artificial enzyme membranes.
- To enable accurate and automated determination of substrate concentrations, exemplified by glucose.
- To establish a robust calibration procedure for reliable measurements.
Main Methods:
- Utilizing an electrode with an artificial enzyme membrane to detect substrate presence.
- Applying a least-square approximation with a third-degree polynomial to transient electrode signals.
- Employing a cubic spline approximation for calibration relating signal slope to substrate concentration.
Main Results:
- The slope at the inflection point of the polynomial approximation is characteristic of the external glucose concentration.
- A calibration procedure was established for automatic measurement of glucose concentrations.
- The system demonstrated the capability for automated sample analysis, including rinsing cycles.
Conclusions:
- A numerical treatment of electrode signals from artificial enzyme membranes can accurately determine substrate concentrations.
- The developed method, using polynomial and spline approximations, allows for automated and precise glucose level measurements.
- This approach enhances the efficiency and reliability of biosensing systems.
More Related Videos
Related Concept Videos
01:19Introduction to Enzyme Kinetics
Enzyme kinetics studies the rates of biochemical reactions. Scientists monitor the reaction rates for a particular enzymatic reaction at various substrate concentrations. Additional trials with inhibitors or other molecules that affect the reaction rate may also be performed.
The experimenter can then plot the initial reaction rate or velocity (Vo) of a given trial against the substrate concentration ([S]) to obtain a graph of the reaction properties. For many enzymatic reactions involving a...
The experimenter can then plot the initial reaction rate or velocity (Vo) of a given trial against the substrate concentration ([S]) to obtain a graph of the reaction properties. For many enzymatic reactions involving a...
01:19Enzyme Kinetics
Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
01:33Enzyme-Linked Immunosorbent Assay
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
01:20Determination of Michaelis Constant and Maximum Elimination Rate
The Michaelis constant (KM) and the theoretical maximum process rate (Vmax) are vital parameters in the Michaelis-Menten equation, central to many biochemical reactions. They provide essential insights into enzyme kinetics and drug metabolism.
These parameters can be estimated by analyzing plasma concentration data post-drug administration. A notable example of this application is phenytoin, a drug with capacity-limited kinetics. It's recommended that phenytoin should be administered at two...
These parameters can be estimated by analyzing plasma concentration data post-drug administration. A notable example of this application is phenytoin, a drug with capacity-limited kinetics. It's recommended that phenytoin should be administered at two...