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Updated: Jul 4, 2026

Creating Rapid Oxygen Oscillations in Microbial Single-cell Growth Analysis using a Microfluidic Double-layer Device
Published on: July 18, 2025
Oxygen electrode dynamics: three-layer model--chemical reaction in the liquid film
1Institute of Chemical Technology, Departement of Chemical Engineering, 16628 Prague 6, Czechoslovakia.
Abstract:
A general model of the transient behaviour of an oxygen probe is derived on more general assumptions than those previously considered. The present state of the art is shortly discussed in its relation to the newly derived equations: (1) the reducibility of the three-layer diffusional model to two-layer ones is discussed, and the two-layer model considering the membrane and the liquid film is verified experimentally, (2) experimental and theoretical treatments of transient probe characteristics determination are examined, and (3) the influence of chemical reactions of the first order as well as of zero order (microorganism respiration) in measured media on transient probe characteristics is quantified.
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