Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jul 4, 2026

Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells

Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells

Published on: November 17, 2017

iTRAQ experimental design for plasma biomarker discovery.

Xiaomin Song¹, Julia Bandow, Jamie Sherman

¹Australian Proteome Analysis Facility Ltd., Macquarie University, Sydney, Australia.

Journal of Proteome Research

|June 14, 2008

Summary

Optimizing experimental design for mass spectrometry-based biomarker discovery in human plasma using iTRAQ reagents is crucial. This study reveals factors influencing quantitative accuracy and reproducibility for robust results.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Prognostic biomarkers of distant metastasis following curative-intent resection of early-stage rectal cancer.

Clinical proteomics·2026

Same author

Visualization of Gas Mass Transfer by Interferometric Microsphere Microscopy.

Analytical chemistry·2026

Same author

Unveiling novel biomarkers for diabetes-related complications through large-scale proteomics analysis: A FIELD sub-study.

Journal of diabetes and its complications·2026

Same author

Ionic liquid-functionalized isomeric covalent organic frameworks for enhanced photocatalytic hydrogen peroxide production under visible-light irradiation.

Journal of colloid and interface science·2026

Same author

External Guidance Incomplete Cross-Modal Hashing.

IEEE transactions on image processing : a publication of the IEEE Signal Processing Society·2026

Same author

ASO Visual Abstract: Circulating Extracellular Vesicle Biomarkers as Diagnostic and Prognostic Biomarkers of Liver Metastasis in Colorectal Cancer: A Systematic Review.

Annals of surgical oncology·2026

Area of Science:

Biochemistry
Proteomics
Analytical Chemistry

Background:

Mass spectrometry is vital for biomarker discovery in human plasma.
iTRAQ reagents enable sample multiplexing and quantitation in large-scale studies.
Optimizing experimental design is key for reliable biomarker identification.

Purpose of the Study:

To investigate experimental design aspects for large-scale plasma biomarker discovery using iTRAQ.
To assess the reproducibility and quantitative variance in iTRAQ experiments.
To evaluate the impact of reference sample choice on quantitative ratio calculations.

Main Methods:

Utilized iTRAQ reagents for multiplexing and quantitation of immunodepleted human plasma samples.
Employed ProteinPilot software for peptide identification and quantitation.

More Related Videos

Isolation, Characterization, and Proteomic Analysis of Plasma-Derived Extracellular Vesicles for Cardiovascular Biomarker Discovery

Isolation, Characterization, and Proteomic Analysis of Plasma-Derived Extracellular Vesicles for Cardiovascular Biomarker Discovery

Published on: January 31, 2025

Related Experiment Videos

Last Updated: Jul 4, 2026

Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells

Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells

Published on: November 17, 2017

Isolation, Characterization, and Proteomic Analysis of Plasma-Derived Extracellular Vesicles for Cardiovascular Biomarker Discovery

Isolation, Characterization, and Proteomic Analysis of Plasma-Derived Extracellular Vesicles for Cardiovascular Biomarker Discovery

Published on: January 31, 2025

Compared plasma from healthy volunteers and osteoarthritis patients across eight iTRAQ experiments.

Main Results:

Achieved excellent run-to-run reproducibility for protein level ratios (CV 11.7%).
Observed significant quantitative variance between peptides for the same protein, linked to lower iTRAQ reporter ion intensity.
Found immunodepletion had a negligible effect on quantitative variance.

Conclusions:

iTRAQ methodology offers good reproducibility for plasma biomarker discovery.
Careful consideration of experimental design, including peptide selection and reference samples, is essential for robust results.
Further optimization can enhance the reliability of plasma-based biomarker discovery using mass spectrometry.