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Published on: December 19, 2025
Optimizing the use of frozen-thawed equine semen
1Equine Medical Center of Ocala, 7107 West Highway 326, Ocala, FL 34482, USA. CMi047690@aol.com
Theriogenology
|June 14, 2008
Summary
Breeding mares with frozen-thawed equine semen can achieve good pregnancy rates. A single insemination dose within 6 hours post-ovulation, with proper mare management, yielded a 45% success rate.
Area of Science:
- Equine reproduction
- Animal breeding
- Veterinary science
Background:
- Frozen-thawed equine semen presents unique challenges in mare reproductive efficiency.
- Optimizing artificial insemination (AI) protocols is crucial for successful equine breeding programs.
Purpose of the Study:
- To review current protocols for breeding mares with frozen-thawed equine semen.
- To evaluate factors influencing pregnancy rates and assess the efficacy of single-dose insemination.
Main Methods:
- Review of existing literature on equine artificial insemination with frozen-thawed semen.
- Retrospective analysis of breeding records to determine pregnancy rates.
- Evaluation of mare selection, ovulation induction, insemination timing, and post-insemination management.
Main Results:
- A single dose of frozen-thawed semen inseminated within 6 hours post-ovulation resulted in a 45% pregnancy rate (67/149).
- This rate is comparable to previous studies using multiple doses and commercial conditions.
- Key factors influencing success include mare selection, ovulation induction, and precise insemination timing.
Conclusions:
- Acceptable pregnancy rates are achievable with a single dose of frozen-thawed semen per cycle.
- Proper breeding management, including timing insemination within 6 hours post-ovulation, is essential.
- This approach offers a viable option for equine breeding programs utilizing frozen semen.

