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Related Experiment Video

Updated: Jul 4, 2026

Isolation of Leukocytes from the Human Maternal-fetal Interface

Isolation of Leukocytes from the Human Maternal-fetal Interface

Published on: May 21, 2015

Expression and function of PDCD1 at the human maternal-fetal interface.

Elizabeth S Taglauer¹, Ann S Trikhacheva, Joyce G Slusser

¹Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA.

Biology of Reproduction

|June 14, 2008

Summary

Maternal T cells express PDCD1 (Programmed cell death protein 1) during pregnancy. This pathway, via PDCD1:CD274 interaction, modulates immune responses by suppressing specific cytokine production in decidual T cells.

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Last Updated: Jul 4, 2026

Isolation of Leukocytes from the Human Maternal-fetal Interface

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Published on: May 21, 2015

Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae

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Published on: April 30, 2018

Purification of HLA-G+ Extravillous Trophoblasts from Human Term Placental Tissues for Phenotyping and Functional Analysis

Purification of HLA-G⁺ Extravillous Trophoblasts from Human Term Placental Tissues for Phenotyping and Functional Analysis

Published on: March 13, 2026

Area of Science:

Immunology
Reproductive Immunology
Cell Biology

Background:

Maternal T lymphocytes normally tolerate the semiallogenic fetus, indicating potent regulatory mechanisms.
Programmed cell death protein 1 (PDCD1) is a CD28 family receptor on T cells, with its ligand CD274 highly expressed on human placental trophoblast cells.

Purpose of the Study:

To investigate the expression of PDCD1 in human maternal T cells during pregnancy.
To explore the functional consequences of PDCD1:CD274 interactions on decidual T cells.

Main Methods:

Immunofluorescence staining of uterine tissues to detect PDCD1 expression on CD3+ cells.
Quantitative analysis of PDCD1 mRNA isoforms in decidual versus peripheral blood T cells.
Co-culture experiments using CD274-transfected Jar choriocarcinoma cells with decidual T cells to assess cytokine production and apoptosis.

Main Results:

PDCD1 expression on maternal T cells increases from nonpregnant endometrium to first-trimester and term decidua.
Term decidual T cells, including CD4+, CD8 bright, and regulatory T cells, show higher PDCD1 expression and mRNA levels compared to peripheral blood.
PDCD1:CD274 interaction significantly suppressed interferon-gamma and tumor necrosis factor-alpha production by decidual CD4+ T cells, but not CD8 bright T cells or IL10 secretion.

Conclusions:

The PDCD1:CD274 pathway is active in maternal decidual T cells during pregnancy.
This pathway plays a role in modulating maternal immune responses by altering cytokine secretion from decidual lymphocytes, contributing to fetal tolerance.