Related Experiment Video
Updated: Jul 4, 2026

The Benthic Exchange of O2, N2 and Dissolved Nutrients Using Small Core Incubations
Published on: August 3, 2016
Effect of dissolved oxygen on nitrogen fixation by A. vinelandii. I. Free cell cultures
1Department of Chemical Engineering, University of Virginia, Charlottesville, Virginia 22901.
Abstract:
As part of an investigation into the use of biological nitrogen fixation for fertilizer ammonia production, continuous culture studies of respiration and nitrogen fixation in the aerobic bacteria Azotobacter vinelandii under oxygen-limited conditions were conducted. Respiration and growth rates followed Monod forms with respect to dissolved oxygen concentration. However, specific nitrogen fixation rate and nitrogenase activity exhibited maximum values at dissolved oxygen concentrations of ca. 0.02 mM (10% of air saturation). These results suggest careful control of oxygen in the environment is necessary to optimize fixed nitrogen production by this organism.
More Related Videos
09:45Laboratory Simulation of an Iron(II)-rich Precambrian Marine Upwelling System to Explore the Growth of Photosynthetic Bacteria
Published on: July 24, 2016
08:28Creating Rapid Oxygen Oscillations in Microbial Single-cell Growth Analysis using a Microfluidic Double-layer Device
Published on: July 18, 2025
Related Concept Videos
Bioreactor Controls-II
Oxygen Requirements and Growth Patterns
Inorganic Nitrogen Assimilation
Oxygen Transport in the Blood
Microbes and the Nitrogen Cycle
The Winogradsky Column