Related Experiment Video
Updated: Jul 4, 2026

Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies
Published on: January 23, 2019
The growth dynamics of a methanol-utilizing bacterium L3 in a batch bioreactor
1School of Chemical Engineering, Purdue University, West Lafayette, Indiana 47907.
Abstract:
The growth dynamics of a methanol utilizing bacterium, L3, in batch bioreactors were experimentally investigated. Formaldehyde, a key intermediate of methanol oxidation, is indicated to have a significant role in the complex batch growth behavior of L3. The intricate batch growth dynamics of many microorganisms can be elegantly characterized by examining the specific rates of exchange of nutrients and products between the cells and the cellular environment. Following such an analysis, the batch growth of L3 on methanol was characterized by the presence of unbalanced and balanced growth phases. The nature and significance of nutrient and product concentration profiles or semilog-arithmic profiles of nutrient and product exchange rates during balanced and unbalanced growth phases are also outlined.
Related Concept Videos
Scale-Up Processes
Bioreactor Controls-III
Designing Growth Media for Bioreactors
Bioreactor Controls-II
Upstream Processing
Batch vs Continuous Culture

