Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interaction between ionizing radiation and supercoiled DNA within human tumor cells.

A T Vaughan1, A M Milner, D G Gordon

  • 1Department of Immunology, University of Birmingham, United Kingdom.

Cancer Research
|August 1, 1991
PubMed
Summary

Radiosensitive cancer cells exhibit altered DNA supercoiling and nuclear matrix interactions, leading to increased DNA damage complexity after radiation exposure. These structural differences impact DNA repair efficiency.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Workshop on the design and use of clinical trials with multiple endpoints, with a focus on prevention of RSV.

Vaccine: X·2025
Same author

Unraveling the functional attributes of the language connectome: crucial subnetworks, flexibility and variability.

NeuroImage·2022
Same author

Heterogeneous mass distribution of the rubble-pile asteroid (101955) Bennu.

Science advances·2020
Same author

Perspectives from Young South African and Zimbabwean Women on Attributes of Four (Placebo) Vaginal Microbicide Delivery Forms.

AIDS and behavior·2019
Same author

Longitudinal distribution of macroinvertebrates in snowmelt streams in northeast Greenland: understanding biophysical controls.

Polar biology·2019
Same author

A partially differentiated interior for (1) Ceres deduced from its gravity field and shape.

Nature·2016

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Radiation Oncology

Background:

  • DNA supercoiling is crucial for genome organization and function.
  • Nuclear matrix interactions influence DNA structure and accessibility.
  • Understanding radiosensitivity is key for cancer treatment optimization.

Purpose of the Study:

  • To investigate DNA supercoiling differences in radiosensitive versus radioresistant cancer cells.
  • To explore the relationship between DNA supercoiling, nuclear matrix, and radiosensitivity.
  • To assess the impact of gamma radiation on DNA structure in these cell lines.

Main Methods:

  • Analysis of DNA supercoiling in histone-free nuclei (nucleoids) from human squamous cell carcinoma lines.
  • Flow cytometry with ethidium bromide staining to measure DNA fluorescence and light scatter.

Related Experiment Videos

  • Assessment of nucleoid structural changes after gamma irradiation.

    • Main Results:

    • Radiosensitive cells showed resistance to supercoiling transitions and greater variation in ethidium bromide binding.
    • Gamma radiation induced more relaxed supercoiled DNA in radiosensitive cells.
    • Nucleoids from radiosensitive cells were larger post-irradiation, suggesting altered DNA-nuclear matrix interactions.

    Conclusions:

    • Observed structural alterations in radiosensitive cells are likely due to modified DNA-nuclear matrix affinity.
    • These alterations, combined with radiation damage, may result in more complex DNA repair challenges.
    • Findings suggest a link between DNA supercoiling dynamics, nuclear architecture, and cellular radiosensitivity.