Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dermoscopy and trichoscopy in Klippel-Trénaunay syndrome.

Dermatology online journal·2026
Same author

Bullous dermatitis artefacta histopathologically mimicking porphyria cutanea tarda.

Dermatology online journal·2026
Same author

Aedes aegypti salivary gland extract enhances Zika virus replication through immune modulation.

Memorias do Instituto Oswaldo Cruz·2026
Same author

Pemphigus Foliaceus. Reply.

The New England journal of medicine·2026
Same author

A Bead-Based Screening Platform for Identifying Monoclonal Antibodies That Disrupt PD-1/PD-L1 Interactions.

Journal of immunology research·2026
Same author

Mwah: Klimt's The Kiss, other kisses, and the "kiss" in clinical dermatology.

Clinics in dermatology·2026

Related Experiment Video

Updated: Jul 4, 2026

Development and Functional Characterization of Murine Tolerogenic Dendritic Cells
09:51

Development and Functional Characterization of Murine Tolerogenic Dendritic Cells

Published on: May 18, 2018

Alterations in dendritic cell function in aged mice: potential implications for immunotherapy design.

Carine Paula1, Adriana Motta, Carla Schmitz

  • 1Instituto de Pesquisas Biomédicas, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil.

Biogerontology
|June 17, 2008
PubMed
Summary

Aging impairs the bone marrow

More Related Videos

Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes
06:09

Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes

Published on: June 22, 2016

Quantitative Imaging of Lineage-specific Toll-like Receptor-mediated Signaling in Monocytes and Dendritic Cells from Small Samples of Human Blood
07:58

Quantitative Imaging of Lineage-specific Toll-like Receptor-mediated Signaling in Monocytes and Dendritic Cells from Small Samples of Human Blood

Published on: April 16, 2012

Related Experiment Videos

Last Updated: Jul 4, 2026

Development and Functional Characterization of Murine Tolerogenic Dendritic Cells
09:51

Development and Functional Characterization of Murine Tolerogenic Dendritic Cells

Published on: May 18, 2018

Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes
06:09

Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes

Published on: June 22, 2016

Quantitative Imaging of Lineage-specific Toll-like Receptor-mediated Signaling in Monocytes and Dendritic Cells from Small Samples of Human Blood
07:58

Quantitative Imaging of Lineage-specific Toll-like Receptor-mediated Signaling in Monocytes and Dendritic Cells from Small Samples of Human Blood

Published on: April 16, 2012

Area of Science:

  • Immunology
  • Gerontology
  • Cell Biology

Background:

  • Immune system function declines with age, particularly adaptive immunity.
  • Dendritic cells (DCs), key antigen-presenting cells (APCs), may be affected by aging, impacting adaptive immunity.
  • Age-related defects in DC generation or function could contribute to diminished immune responses.

Purpose of the Study:

  • To investigate the impact of aging on bone marrow's ability to generate dendritic cells (DCs) in vitro.
  • To assess the functional responses of aged bone marrow-derived DCs to lipopolysaccharide (LPS) and tumor antigens.
  • To evaluate the role of aged DCs in anti-tumor therapies, focusing on phagocytosis and antigen presentation.

Main Methods:

  • Comparison of DC generation from young and aged mouse bone marrow (BM) in vitro.
  • Assessment of BM-derived DC maturation and cytokine production (TNF-alpha) in response to LPS.
  • Evaluation of aged DC phagocytosis of tumor cells and subsequent antigen presentation.
  • Analysis of MHC class II expression on DCs after phagocytosis.

Main Results:

  • Aged BM yielded fewer DCs in vitro compared to young BM.
  • LPS-induced DC maturation was reduced in aged DCs, yet TNF-alpha production was elevated even without LPS.
  • Phagocytosis of tumor cells was unaffected by age, but aged DCs showed decreased tumor antigen presentation.
  • Similar MHC class II upregulation suggested an age-associated defect in exogenous antigen processing.

Conclusions:

  • Aging bone marrow produces fewer mature dendritic cells (DCs) and exhibits reduced responses to LPS.
  • Aged DCs maintain phagocytic capacity but display impaired antigen processing and presentation, crucial for anti-tumor immunity.
  • These findings highlight age-related limitations in DC function relevant for optimizing DC-based vaccine design in older populations.