Oxidative assimilation treatment of a nitrogen-deficient toxic waste

Batch growth tests were performed under both replicating and nonproliferating (no nitrogen source in medium) conditions with acclimated heterogenous populations that utilized phenol as a sole source of carbon and energy. It was shown that the acclimated populations could efficiently remove the toxic waste component phenol under nonproliferating conditions by utilizing an oxidative assimilation mechanism. The phenol was assimilated and converted into nonnitrogenous storage products. During the assimilation process, the cells had a tendency to excrete some product (nonsubstrate) chemical oxygen demand (COD). Bench-scale oxidative assimilation units were operated by sequentially feeding a carbon source (phenol) and nitrogen source (ammonium sulfate) to heterogeneous populations. This demonstrated that, subsequent to the addition of the nitrogen source to the medium, the cells utilized the stored carbon for replication. Four of these units were operated at different phenol COD-to-ammonia-nitrogen ratios of 10:1, 20:1, 40:1, and 50:1. All of these units demonstrated excellent removal of phenol using an oxidative assimilation mechanism. These results suggested the feasibility of utilizing a continuous flow oxidative assimilation process for the treatment of nitrogen-deficient phenolic wastes. This process would be advantageous over conventional treatment processes in that it would realize a savings in chemical costs (ammonia as nitrogen source) and prevent leakage of excess ammonia from the system.