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Invertase covalent grafting onto corn stover
P Monsan1, D Combes, I Alemzadeh
1Départment de Génie Biochimique et Alimentaire, ERA-CNRS No 879, Institut National des Sciences Appliquées, Avenue de Rangueil, F-31077 Toulouse Cédex, France.
Biotechnology and Bioengineering
|July 1, 1984
Summary
Researchers developed a method to immobilize yeast invertase onto corn grits, achieving high enzyme activity and stability. This cost-effective approach offers a 25-fold increase in activity compared to silica supports.
Area of Science:
- Biotechnology
- Enzyme immobilization
- Agricultural by-products
Background:
- Enzyme immobilization is crucial for industrial applications.
- Agricultural by-products offer sustainable and cost-effective support materials.
- Invertase is a key enzyme in carbohydrate processing.
Purpose of the Study:
- To develop a covalent coupling method for immobilizing yeast invertase onto corn grits.
- To optimize the chemical modification steps for the corn grits support.
- To evaluate the activity and stability of the immobilized invertase.
Main Methods:
- Chemical modification of corn grits including oxidation, amination, reduction, and activation.
- Covalent coupling of invertase using optimized conditions.
- Activity assays measuring reducing sugar production.
- Operational stability tests at elevated temperatures and substrate concentrations.
Main Results:
- Achieved high enzyme activity of up to 7.2 x 10(4) mumol reducing sugars/min/g support.
- Immobilized invertase on corn grits showed 25 times higher activity than on porous silica.
- Demonstrated high operational stability with a half-life of 365 days at 40°C in 2M sucrose.
- Successfully scaled up the immobilization process to 10 kg.
Conclusions:
- Corn grits are an effective and superior support for invertase immobilization compared to silica.
- The developed method provides a highly active, stable, and scalable enzyme derivative.
- This approach offers a sustainable and economical solution for industrial enzyme applications.
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