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Related Experiment Video

Updated: Jul 4, 2026

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

Published on: October 24, 2016

Ethanol fermentation in a continuous tower fermentor.

S T Jones¹, R A Korus, W Admassu

¹Department of Chemical Engineering, Washington State University, Pullman, Washington 99164, USA.

Biotechnology and Bioengineering

|July 1, 1984

Summary

This summary is machine-generated.

A flocculating yeast mutant rapidly fermented high glucose concentrations in a continuous tower fermentor. This process achieved high ethanol productivity and conversion, demonstrating efficient industrial biotechnology potential.

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Area of Science:

Biotechnology
Biochemical Engineering
Microbiology

Background:

Saccharomyces cerevisiae is a key microorganism for industrial ethanol production.
High glucose concentrations can inhibit yeast fermentation, reducing efficiency.
Developing robust yeast strains and fermentation processes is crucial for optimizing ethanol yields.

Purpose of the Study:

To evaluate the fermentation performance of a flocculating Saccharomyces cerevisiae cerevisiae mutant.
To assess the efficiency of a continuous nonaerated tower fermentor for high-gravity ethanol production.
To model the fermentation kinetics, considering ethanol inhibition and glucose dependence.

Main Methods:

Utilized a flocculating Saccharomyces cerevisiae mutant in a continuous nonaerated tower fermentor at 30°C.
Operated the fermentor continuously for seven months with high glucose concentrations (100-180 g/L).
Applied a kinetic model incorporating linear ethanol inhibition and Monod kinetics for data analysis.

Main Results:

The yeast mutant rapidly fermented high glucose media, achieving >90% of theoretical conversion.
Ethanol productivities of 8-12 g/L/h were attained within the yeast bed.
Fermentation performance was correlated with apparent fermentation time and initial glucose levels.

Conclusions:

Flocculating Saccharomyces cerevisiae mutants are highly effective for high-gravity ethanol fermentation.
Continuous tower fermentation offers a viable and efficient method for industrial ethanol production.
The developed kinetic model accurately describes fermentation under these conditions, aiding process optimization.