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Determining the inhibition constants in the HCH-1 model of cellulose hydrolysis
M T Holtzapple1, H S Caram, A E Humphrey
1US Army Natick R & D Laboratories, Natick, Massachusetts 01760, USA.
Biotechnology and Bioengineering
|July 1, 1984
Abstract:
The products of cellulose hydrolysis, glucose and cellobiose, caused noncompetitive inhibition patterns to be exhibited when Thermomonospora sp. YX cellulase hydrolyzed dyed cellulose. The glucose binding constant, beta(1), was 0.00683 +/- 0.00031 L/g and the cellobiose binding constant, beta(2), was 0.095 +/- 0.0058 L/g. Thus, cellobiose was about 14 times more inhibitory than glucose.
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