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Updated: Jul 4, 2026

High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits
Published on: September 20, 2016
The HCH-1 model of enzymatic cellulose hydrolysis
M T Holtzapple1, H S Caram, A E Humphrey
1US Army Natick R&D Laboratories, Natick, Massachusetts 01760, USA.
Abstract:
Solka Floc BW200 was enzymatically hydrolyzed in a batch reactor using a commercial cellulase preparation. A total of 50 different hydrolysis conditions were run within a 10-fold range in enzyme concentration and a 30-fold range in cellulose concentration. The data were evaluated in three ways using five different models. Previous literature models were not as successful in correlating the data as the HCH-1 Model derived in this work.
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