Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Production of Alcohol01:27

Production of Alcohol

Continuous fermentation is a key strategy in industrial ethanol production, particularly when efficiency, scalability, and high yields are essential. This approach allows for uninterrupted operation and optimized resource utilization. The primary feedstock, corn starch, undergoes enzymatic hydrolysis facilitated by α-amylase and glucoamylase. These enzymes break down the starch into fermentable sugars such as glucose, which are readily assimilated by fermentative microorganisms.Fermentation...
Bioreactor Controls-III01:22

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
Microbial Fermentation01:23

Microbial Fermentation

Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
Fermentation01:29

Fermentation

Most eukaryotic organisms require oxygen to survive and function adequately. Such organisms produce large amounts of energy during aerobic respiration by metabolizing glucose and oxygen into carbon dioxide and water. However, most eukaryotes can generate some energy in the absence of oxygen by anaerobic metabolism.
Fermentation is a type of metabolic process that occurs in the absence of oxygen, where organic molecules such as glucose are broken down to produce energy. During this process, the...
Microbes in Beverage Production01:25

Microbes in Beverage Production

Alcoholic beverages such as wine, beer, and spirits are the products of microbial fermentation processes that transform simple sugars into ethanol and a wide array of complex flavor compounds. These transformations rely on the metabolic activities of specific yeasts and bacteria, which are selected and controlled to yield the desired beverage characteristics.Wine Fermentation and MaturationWine production begins with the crushing of grapes to release juice and pulp, forming a must that is...
Bioreactor Controls-II01:18

Bioreactor Controls-II

In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Morphological Study of Carotid Artery of WHHL Rabbit after Balloon Dilatation.

Interventional neuroradiology : journal of peritherapeutic neuroradiology, surgical procedures and related neurosciences·2010
Same author

Embolization of scalp AVF.

Interventional neuroradiology : journal of peritherapeutic neuroradiology, surgical procedures and related neurosciences·2010
Same author

Rupture of a large vertebral artery aneurysm following proximal occlusion.

Interventional neuroradiology : journal of peritherapeutic neuroradiology, surgical procedures and related neurosciences·2010
Same author

Endovascular trapping for a giant aneurysm of the cervical internal carotid artery: a case report.

Interventional neuroradiology : journal of peritherapeutic neuroradiology, surgical procedures and related neurosciences·2010
Same author

Quantitative lymph imaging for assessment of lymph function using indocyanine green fluorescence lymphography.

European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery·2008
Same author

Indocyanine green fluorescence angiography for intraoperative assessment of blood flow: a feasibility study.

European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery·2007
Same journal

Developing Anti-EGFR/Anti-HER2 Bifunctional Antibody for Solid Tumors by Protein Engineering.

Biotechnology and bioengineering·2026
Same journal

Bridging Organ-on-a-Chip and Omics: A Multi-Dimensional Frontier in Biomedical Research.

Biotechnology and bioengineering·2026
Same journal

Hemopexin Purification From Human Cohn Fraction IV Paste and Its Biophysical Characterization and Functional Evaluation in Sickle Cell Disease Mice.

Biotechnology and bioengineering·2026
Same journal

Characterization and Therapeutic Potential of a Novel Lytic Phage-Derived Endolysin PA16cLys Against Uropathogenic Pseudomonas aeruginosa Biofilms.

Biotechnology and bioengineering·2026
Same journal

Modeling Multiscale Architecture of Biofilm Extracellular Matrix and Its Role in Oxygen Transport.

Biotechnology and bioengineering·2026
Same journal

A Behavior-Integrated Potency Assay for Quantitative Evaluation of Extracellular Matrix Remodeling by Mesenchymal Stem/Stromal Cells.

Biotechnology and bioengineering·2026
See all related articles

Related Experiment Video

Updated: Jul 4, 2026

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
14:53

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

Published on: October 24, 2016

Continuous ethanol fermentation using immobilized yeast cells.

M Nagashima1, M Azuma, S Noguchi

  • 1Technical Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Hofu Plant, Kyowa-cho 1-1, Hofu-shi, Yamaguchi-ken 747, Japan.

Biotechnology and Bioengineering
|August 1, 1984
PubMed
Summary
This summary is machine-generated.

Immobilized Saccharomyces cerevisiae cells in bioreactors achieved high ethanol productivity and stability for over six months. Enhancements like sterol addition boosted performance in continuous fermentation from cane molasses.

More Related Videos

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production
10:10

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Published on: September 20, 2016

A Rapid Technique for the Visualization of Live Immobilized Yeast Cells
02:54

A Rapid Technique for the Visualization of Live Immobilized Yeast Cells

Published on: November 9, 2006

Related Experiment Videos

Last Updated: Jul 4, 2026

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
14:53

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

Published on: October 24, 2016

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production
10:10

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Published on: September 20, 2016

A Rapid Technique for the Visualization of Live Immobilized Yeast Cells
02:54

A Rapid Technique for the Visualization of Live Immobilized Yeast Cells

Published on: November 9, 2006

Area of Science:

  • Biotechnology
  • Biochemical Engineering
  • Microbiology

Background:

  • Continuous ethanol fermentation using immobilized yeast cells offers potential for industrial applications.
  • Maintaining cell viability and preventing contamination are critical challenges for long-term fermentation processes.
  • Optimizing bioreactor conditions can significantly enhance ethanol yield and productivity.

Purpose of the Study:

  • To develop a stable and highly productive continuous ethanol fermentation process using immobilized Saccharomyces cerevisiae.
  • To investigate methods for improving cell viability and preventing microbial contamination in immobilized cell bioreactors.
  • To evaluate the performance of a pilot-scale fluidized-bed reactor for ethanol production from cane molasses.

Main Methods:

  • Immobilization of Saccharomyces cerevisiae cells in calcium alginate gel beads.
  • Utilization of fluidized-bed reactors for continuous fermentation of cane molasses.
  • Incorporation of sterols and unsaturated fatty acids into immobilized beads to enhance cell stability and productivity.
  • Monitoring of ethanol concentration, cell viability, and microbial contamination over extended operational periods.

Main Results:

  • Ethanol productivity exceeding 50 g ethanol/L gel/h was achieved with enhanced immobilized beads.
  • Stable operation exceeding one-half year was maintained, with cell concentrations over 250 g dry cell/L gel.
  • A pilot plant (4 kL) continuously produced 8-10% (v/v) ethanol from non-sterilized cane molasses for over six months.
  • Ethanol productivity reached 0.6 kL ethanol/kL reactor volume/day with 95% conversion yield.

Conclusions:

  • Immobilization of Saccharomyces cerevisiae in calcium alginate, enhanced with sterols and fatty acids, provides a robust method for continuous ethanol fermentation.
  • The fluidized-bed reactor system demonstrates high efficiency and long-term stability for industrial-scale bioethanol production.
  • This technology offers a viable solution for sustainable bioethanol production from sugar sources like cane molasses.