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Related Concept Videos

Affinity Chromatography01:03

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
Silica Gel Column Chromatography: Overview01:10

Silica Gel Column Chromatography: Overview

Silica gel column chromatography is a technique for separating compounds using a column packed with silica gel as the stationary phase. This method relies on differences in the polarity of compounds. Based on their polarities, compounds move between the stationary phase (silica gel) and the mobile phase (the solvent), forming discrete bands in the column.
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
Chromatography: Introduction01:10

Chromatography: Introduction

Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
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Analyte Adsorption and Distribution

In certain chromatographic separations, solutes transfer between the mobile phase and the stationary phase via sorption, which typically refers to the process of adsorption. For many chromatographic systems, the sorption process often depends on the polarity of the compounds—an expression of the overall dipole moment within the molecule. During the separation process, there is competition between the solute and solvent for adsorption to the stationary phase. Highly polar compounds and solvents...

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Affinity Purification of a 6X-His-Tagged Protein using a Fast Protein Liquid Chromatography System
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Affinity Purification of a 6X-His-Tagged Protein using a Fast Protein Liquid Chromatography System

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Semicontinuous affinity chromatography separations.

B J McCoy1

  • 1Department of Chemical Engineering, University of California, Davis, California 95616.

Biotechnology and Bioengineering
|October 1, 1985
PubMed
Summary
This summary is machine-generated.

This study mathematically models semicontinuous affinity chromatography. Cyclic operation with alternating adsorption and desorption periods offers significant advantages for separations.

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Area of Science:

  • Chemical Engineering
  • Separation Science

Background:

  • Affinity chromatography is a powerful separation technique.
  • Optimizing column operation is crucial for efficiency.

Purpose of the Study:

  • To mathematically investigate the semicontinuous operation of affinity chromatography columns.
  • To analyze periodic adsorption and desorption cycles.
  • To determine the optimal time intervals for enhanced separation.

Main Methods:

  • Mathematical modeling of semicontinuous affinity chromatography.
  • Utilizing a nonlinear adsorption equilibrium relation.
  • Solving governing differential equations via the method of characteristics.

Main Results:

  • The study provides a mathematical framework for semicontinuous column operation.
  • Estimation of optimal time intervals between adsorption and desorption phases.
  • Demonstration of improved separation efficiency through cyclic operation.

Conclusions:

  • Semicontinuous cyclic operation of affinity chromatography columns is advantageous for separations.
  • Mathematical analysis provides insights into optimizing adsorption and desorption cycles.
  • This approach enhances the efficiency of chromatographic separations.