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Assay of the high-alkaline proteinase from alkalophilic bacillus PB92 using a chromogenic tripeptide substrate
C J Gray1, S A Barker, M S Dhariwal
1Department of Chemistry, University of Birmingham, P.O. Box 363 Birmingham, B15 2TT, United Kingdom.
Biotechnology and Bioengineering
|December 1, 1985
Abstract:
The chromogenic tripeptide substrate, benzyloxycarbonylglycyl-L-prolyl-L-citrulline p-nitroanilide, is proposed for the assay of the high-alkaline proteinase, HAP-PB92, from an alkalophilic Bacillus. The assay method is sensitive, reproducible, and may be adapted for an automatic system.
